Computer-assisted detection of monoclonal components: results from the multicenter study for the evaluation of CASPER (Computer Assisted Serum Protein Electrophoresis Recognizer) algorithm

Clinical Chemistry and Laboratory Medicine : CCLM
Agostino OgnibeneGianni Messeri

Abstract

To investigate the potential use of Artificial Neural Network (ANN) in the evaluation of serum protein electrophoresis, we set up a multicenter study involving six Italian laboratories. For this purpose, we developed an algorithm named CASPER (Computer Assisted Serum Protein Electrophoresis Recognizer). A total of 59,516 samples from the six centers were divided into three groups. Training and validation sets were used to develop the neural network, whereas evaluation set was used to test the performance of CASPER in recognizing abnormal electrophoretic profiles. CASPER showed 93.0% sensitivity and 47.4% specificity. CASPER sensitivity and specificity ranged in the six sites from 88% (site 3) to 97% (site 5) and from 36% (site 6) to 53% (site 3), respectively. Sensitivity for gamma zone was 94.6%, for beta zone 89.7% and for oligoclonal patterns 92.0%. The sensitivity of the CASPER algorithm does not allow us to recommend its use as a replacement for the visual inspection, but it could be helpful in avoiding accidental misclassifications by the operator. Moreover, the CASPER algorithm may be a useful tool for training operators and students. This study evidenced a high inter-observer variability, which should be addressed in a ...Continue Reading

References

Jul 1, 1992·Journal of Clinical Pathology·M A KratzerA Fateh-Moghadam
Apr 1, 1992·European Journal of Haematology·F AguzziG Merlini
Mar 2, 1999·Archives of Pathology & Laboratory Medicine·R AlexanianF Liu
Mar 6, 2003·Reviews in Clinical and Experimental Hematology·Robert A Kyle, S Vincent Rajkumar
Oct 28, 2003·Clinical Chemistry·Cécile Gay-BellileThierry Le Bricon
Dec 4, 2004·Clinical Chemistry and Laboratory Medicine : CCLM·Agostino OgnibeneGianni Messeri
Mar 31, 2006·The New England Journal of Medicine·Robert A KyleL Joseph Melton
Aug 30, 2006·British Journal of Haematology·Robert A Kyle, S Vincent Rajkumar

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