Computer-assisted image analysis of human cilia and Chlamydomonas flagella reveals both similarities and differences in axoneme structure.

Cytoskeleton
Eileen T O'TooleLawrence E Ostrowski

Abstract

In the past decade, investigations from several different fields have revealed the critical role of cilia in human health and disease. Because of the highly conserved nature of the basic axonemal structure, many different model systems have proven useful for the study of ciliopathies, especially the unicellular, biflagellate green alga Chlamydomonas reinhardtii. Although the basic axonemal structure of cilia and flagella is highly conserved, these organelles often perform specialized functions unique to the cell or tissue in which they are found. These differences in function are likely reflected in differences in structural organization. In this work, we directly compare the structure of isolated axonemes from human cilia and Chlamydomonas flagella to identify similarities and differences that potentially play key roles in determining their functionality. Using transmission electron microscopy and 2D image averaging techniques, our analysis has confirmed the overall structural similarity between these two species, but also revealed clear differences in the structure of the outer dynein arms, the central pair projections, and the radial spokes. We also show how the application of 2D image averaging can clarify the underlying st...Continue Reading

References

Sep 1, 1992·The Journal of Cell Biology·D N MastronardeM E Porter
Sep 1, 1992·Molecular and Cellular Biology·A M CurryJ L Rosenbaum
May 1, 1991·The Journal of Cell Biology·H SakakibaraR Kamiya
Jan 1, 1988·Cell Motility and the Cytoskeleton·A T HastieJ S Wall
Jan 1, 1995·Methods in Cell Biology·E O'TooleM E Porter
Jun 1, 1994·The Journal of Cell Biology·M BernsteinJ L Rosenbaum
Jan 1, 1996·American Journal of Respiratory Cell and Molecular Biology·T E GrayP Nettesheim
Dec 1, 1996·American Journal of Respiratory and Critical Care Medicine·A WannerT G O'Riordan
Apr 1, 1999·American Journal of Respiratory Cell and Molecular Biology·L E OstrowskiP Nettesheim
Jun 13, 2001·The Journal of Cell Biology·P YangW S Sale
Aug 10, 2002·Molecular & Cellular Proteomics : MCP·Lawrence E OstrowskiRichard C Boucher
Oct 31, 2002·American Journal of Respiratory and Critical Care Medicine·Estelle EscudierAndré Coste
Dec 12, 2002·Molecular Biology of the Cell·Patricia L KultgenSharon L Milgram
Feb 7, 2003·The European Respiratory Journal·T WodehouseP J Cole
Apr 30, 2003·Human Molecular Genetics·Brian E Davy, Michael L Robinson
Dec 6, 2003·American Journal of Respiratory and Critical Care Medicine·Peadar G NooneMichael R Knowles
Jun 15, 2004·Molecular Biology of the Cell·Ramila S Patel-KingStephen M King
Aug 5, 2004·Journal of Cell Science·Hui Zhang, David R Mitchell
Dec 2, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ruth YokoyamaDavid R Mitchell
Mar 8, 2005·American Journal of Respiratory and Critical Care Medicine·Manfred FliegaufHeymut Omran

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Citations

Sep 1, 2012·American Journal of Respiratory Cell and Molecular Biology·Thomas BurgoyneAmelia Shoemark
Feb 19, 2013·American Journal of Respiratory Cell and Molecular Biology·Maria Eugenia TevesJerome F Strauss
Jan 3, 2013·Cytoskeleton·Blanca I Carbajal-GonzálezDaniela Nicastro
Oct 30, 2014·Journal of Medical Genetics·Małgorzata KurkowiakMichał Witt
Sep 25, 2016·Cold Spring Harbor Perspectives in Biology·David R Mitchell
Sep 17, 2015·Human Reproduction·Johanna RaidtHeymut Omran
Nov 29, 2013·Cilia·Karl-Ferdinand LechtreckGeorge B Witman
Aug 3, 2017·Cold Spring Harbor Perspectives in Biology·Rasagnya ViswanadhaMary E Porter
Sep 5, 2019·Molecular Biology of the Cell·Jianfeng LinMary E Porter
Mar 10, 2020·FEMS Microbiology Reviews·Morgan BeebyDavid R Mitchell
Sep 20, 2018·American Journal of Respiratory Cell and Molecular Biology·Joffrey MiannéJohn De Vos
Jan 18, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Fan YangMaureen Wirschell
Nov 20, 2016·Cold Spring Harbor Perspectives in Biology·Ximena M Bustamante-Marin, Lawrence E Ostrowski
Sep 12, 2020·Cellular and Molecular Life Sciences : CMLS·Lance Lee, Lawrence E Ostrowski
Oct 1, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Amelia ShoemarkThomas Burgoyne

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