Computer-assisted image analysis of neovascularization in thyroid neoplasms from dogs

American Journal of Veterinary Research
Michael S KentBruce R Madewell

Abstract

To develop a computer-assisted image analysis procedure for quantitation of neovascularization in formalin-fixed paraffin-embedded specimens of thyroid gland tissue from dogs with and without thyroid gland neoplasia. 47 thyroid gland carcinomas, 8 thyroid gland adenomas, and 8 specimens of thyroid tissue from dogs without thyroid gland abnormalities (normal). Serial tissue sections were prepared and stained with antibodies against human CD31 or factor VIII-related antigen (factor VIII-rag). The areas of highest vascularity were identified in CD31-stained sections, and corresponding areas were then identified in factor VIII-rag-stained sections. Image analysis was used to calculate the total vascular density in each section, and neovascularization, expressed as a percentage, was determined as the absolute value of the total vascular density derived from factor VIII-rag-stained sections minus the vascular density derived from CD31-stained sections. Mean vascular density of thyroid gland carcinomas derived from CD31-stained sections was significantly greater than density derived from factor VII I-rag-stained sections. This incremental difference was presumed to represent degree of neovascularization. However, significant differenc...Continue Reading

References

Jan 3, 1991·The New England Journal of Medicine·N WeidnerJ Folkman
Mar 1, 1995·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·G Gasparini, A L Harris
Jan 1, 1995·Surgery Today·A TsuchiyaR Abe
May 1, 1997·Lancet·A L Harris
Jun 1, 1997·The Journal of Pathology·H RogatschH Feichtinger
Oct 23, 1997·The American Journal of Dermatopathology·T OlivryP F Moore
Mar 21, 1998·Journal of the American Animal Hospital Association·L S FinemanP Bonney
Sep 28, 1999·Journal of Veterinary Internal Medicine·J C Graham, R K Myers
Nov 5, 1999·The Journal of Pathology·J A BeliënJ P Baak
Dec 3, 1999·Journal of Magnetic Resonance Imaging : JMRI·J S TaylorN Mayr
Apr 12, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M W Lingen
Aug 5, 2000·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·J H Jensen, R Chandra
Sep 23, 2000·Nature·P Carmeliet, R K Jain
Oct 13, 2000·The Journal of Endocrinology·J D Ramsden
Apr 6, 2001·Baillière's Best Practice & Research. Clinical Endocrinology & Metabolism·M J Schlumberger, M Torlantano

❮ Previous
Next ❯

Citations

Jun 23, 2012·Journal of the American Veterinary Medical Association·Joanne L TuohyStephen J Withrow
Jul 10, 2007·The Veterinary Clinics of North America. Small Animal Practice·Lisa G Barber
Jul 10, 2007·The Veterinary Clinics of North America. Small Animal Practice·MaryAnn G Radlinsky
Jun 27, 2007·Clinical Techniques in Small Animal Practice·Julius M Liptak
Jan 31, 2009·Veterinary and Comparative Oncology·J M LiptakS J Withrow
Jan 6, 2011·Veterinary Surgery : VS·Kei HayashiSusan M Stover
Feb 6, 2020·Veterinary and Comparative Oncology·Sabina Sheppard-OlivaresRaelene M Wouda
Feb 23, 2020·Veterinary Pathology·Bardes B HassanThomas J Rosol
Jun 10, 2020·Veterinary and Comparative Oncology·Ber-In LeeMary-Keara Boss
May 1, 2019·Journal of the American Veterinary Medical Association·Maxime LorangeJulius M Liptak
Jun 9, 2020·Journal of Comparative Pathology·L M C SoaresC A Pescador

❮ Previous
Next ❯

Related Concepts

Related Feeds

Angiogenesis Inhibitors to Treat Cancer

Cancer treatments including angiogenesis inhibitors prevent tumor cells from receiving nutrients and oxygen. Here is the latest research on angiogenesis inhibitors for the treatment of cancer.

Related Papers

The Veterinary Clinics of North America. Small Animal Practice
MaryAnn G Radlinsky
Veterinary Radiology & Ultrasound : the Official Journal of the American College of Veterinary Radiology and the International Veterinary Radiology Association
L PackH D Dookwah
© 2022 Meta ULC. All rights reserved