Computer assisted three-dimensional reconstruction of brain regions from serial section digitized images. Application to the organization of striato-nigral relationships in the rat

Journal of Neuroscience Methods
S RoeschY Maurin

Abstract

We have developed a software which allows the three-dimensional reconstruction of brain regions from serial section digitized images. This software, which generates wire-frame three dimensional models, requires at least a 486 PC microcomputer running Microsoft Windows (3.x or 95). Mosaics of high resolution images, covering large brain areas, digitized by means of a camera fitted on a microscope equipped with a motorized stage, are handled by our software as single high resolution images. Serial sets of such images may be segmented and manually aligned. We have utilized this software to study the organization of striatal efferences within the substantia nigra pars reticulata, as well as the distribution of neuronal cell bodies within the substantia nigra pars compacta after micro-ionophoretic application of wheat germ agglutinin conjugated to horseradish peroxidase into the orofacial sensorimotor region of the striatum. The three dimensional representation of anterogradely labeled striatal efferences confirmed and determined the lamellar organization previously postulated from serial plane section micrographs. The distribution in the rat brain of retrogradely labeled nigro-striatal cell bodies, which had not yet been studied af...Continue Reading

References

Sep 1, 1990·Computerized Medical Imaging and Graphics : the Official Journal of the Computerized Medical Imaging Society·B A Allen, C Levinthal
Nov 1, 1988·Journal of Neuroscience Methods·L S Hibbard, R A Hawkins
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Citations

Jul 8, 2009·Journal of Autism and Developmental Disorders·Axel SchleicherKarl Zilles
Apr 23, 2004·Journal of Neuroscience Methods·Bradley S Duerstock
Jun 1, 2005·Journal of Neuroscience Methods·Philippe Andrey, Yves Maurin
Mar 17, 1999·Brain Research Bulletin·D A McRitchieG M Halliday
Sep 29, 2000·Computerized Medical Imaging and Graphics : the Official Journal of the Computerized Medical Imaging Society·B S DuerstockR B Borgens
Mar 18, 2010·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Bradley S DuerstockBartek Rajwa

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