PMID: 9181304Jun 1, 1997Paper

Computer-based technique for cell aggregation analysis and cell aggregation in in vitro chondrogenesis

Cytometry
I MartinF Beltrame

Abstract

No quantitative methods are currently available to measure different aggregation parameters in cell cultures. In this paper we describe a computer-based technique for the automatic and reliable analysis of cellular aggregates, starting from optical microscopy images of living cells grown in suspension. The method allows determination, on the same sample at different time intervals, of quantitative parameters, including aggregation percentage, average number of cells in aggregates, and aggregate size statistical distribution. To determine the number of cells in an aggregate starting from its two-dimensional microscopic profile, a model has been proposed and verified, using sphere packing theory. Algorithms have been tested on chondrocyte suspension cultures, where cell aggregation is a very early and critical event leading to cell differentiation. Using this technique for the analysis of chick embryo chondrocyte cultures, we observed that aggregate size and development kinetics depend on the culture conditions used. The method, with minor adaptations, is of potential use also in other cell systems to evaluate aggregation indexes or to study aggregation kinetics.

Citations

Aug 30, 2008·Applied and Environmental Microbiology·Richard J ThomasRichard W Titball
Aug 13, 2005·Molecular Membrane Biology·Despina BazouW Terence Coakley
Jan 16, 2008·Expert Opinion on Biological Therapy·Vladimir MironovGlenn D Prestwich
Jul 14, 2009·Biomaterials·Nicolas C RivronClemens A Van Blitterswijk
Apr 28, 2009·Journal of Bioscience and Bioengineering·Masahiro Kino-OkaMasahito Taya
Oct 30, 2004·Biotechnology and Bioengineering·Ericka M BuenoGilda A Barabino
Oct 29, 2005·Biotechnology and Bioengineering·Carlota Diaz Sanchez-BustamanteMartin Fussenegger
Jan 18, 2012·Cells, Tissues, Organs·Allison P Van WinkleMichael S Kallos
Dec 21, 2016·Biotechnology Journal·Ang-Chen TsaiTeng Ma
Jun 27, 2009·Cell Biology International·A H M Khurshid AlamToshifumi Tsukahara
Mar 24, 2017·Journal of the Royal Society, Interface·David A HormuthThomas E Yankeelov
Jan 27, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Guillaume FrascaClaire Wilhelm
Dec 15, 2006·Analytical Chemistry·Amanda J HaesGreg E Collins

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