PMID: 3747518Aug 1, 1986Paper

Computer modeling of estradiol interactions with the estrogen receptor

Journal of Steroid Biochemistry
M S Gordon, A C Notides

Abstract

Two computer models for the binding of estradiol to estrogen receptors were constructed, based solely upon the thermodynamic constraints of the most likely equilibria involved and known equilibrium constants. Previous data had suggested that the positive cooperativity of the system was dependent upon a monomer-dimer equilibrium (Notides et al., Proc. natn. Acad. Sci., U.S.A. 78 (1981) 4926-4930). Using computer modeling, we confirmed that the thermodynamic constraints of a monomer-dimer equilibrium system result in convex Scatchard plots in agreement with experimental data, including the progression to linearity at low receptor concentrations. This technique yielded estimates of the equilibrium constant for dimerization (approx. 10(10) to 10(14) M-1). The dose-response characteristics of the monomer-dimer equilibrium system revealed steep dose-response curves that were sensitive to the receptor concentration. In contrast, the dose-response curves that did not undergo a monomer-dimer equilibrium system and had a single step equilibrium process were more gradual.

References

Feb 13, 1975·Biochimica Et Biophysica Acta·S Nielsen, A C Notides
Mar 1, 1975·Journal of Steroid Biochemistry·A C Notides, S Nielsen
Oct 1, 1975·Steroids·G C Chamness, W L McGuire
Jul 13, 1976·Biochemistry·L W Nichol, D J Winzor
Feb 23, 1984·Nature·W V WelshonsJ Gorski
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A C NotidesD E Hamilton

❮ Previous
Next ❯

Citations

Jan 1, 1989·Journal of Steroid Biochemistry·M Kaufman, L Pinsky
Feb 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·J W TurnerT S Ruh
Mar 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·K Dahlman-WrightJ A Gustafsson
Aug 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·S Dove, H Schönenberger
Apr 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·K Dahlman-WrightJ Carlstedt-Duke
Feb 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·S de LauzonN Cittanova
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·C NerviA M Jetten
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S E FawellM G Parker
Jan 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A M DeMarzoD P Edwards
Dec 24, 1987·Cell·V KumarP Chambon
Jan 1, 1993·Journal of Receptor Research·R N PorrelliD Rodbard
Apr 25, 1997·The Journal of Biological Chemistry·B J CheskisC R Lyttle
May 10, 2002·Environmental Science & Technology·Vered Granek, Judith Rishpon
May 26, 2001·The Journal of Organic Chemistry·G B JonesM A Brown

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.