Computer software for testing drug susceptibility of malaria parasites

Cytometry
P P ReindersH J Tanke

Abstract

A computer program is described for the automated analysis of data obtained by flow cytometry for in vitro antimalarial drug susceptibility testing. Samples of malaria-infected red blood cells (RBC), which were cultured in the presence of different concentrations of antimalarial drugs, were stained with Hoechst. The Hoechst fluorescence intensity of infected RBC corresponds to DNA content of the parasites and to their stage of development. After measurement of the samples by a FACStar flow cytometer equipped with a UV laser and an autosampler, FCS 1.0 data files were generated. The HP PAS-CAL program developed for these files identifies five different populations--uninfected RBC, infected RBC, free parasites, leukocytes, and debris--on the basis of their light scatter and fluorescence characteristics. The program calculates the percentage of infected cells, the total number of parasite nuclei, and the average number of nuclei per parasite. The results of each culture are presented as a drug dose-response curve. During data analysis, user interaction is limited to selecting the first file of the first culture. The algorithm then processes each culture automatically. Potential problems or difficulties in analysis are flagged. To ...Continue Reading

References

Jun 1, 1979·Parasitology·R J Howard, F L Battye
Dec 1, 1979·Antimicrobial Agents and Chemotherapy·R E DesjardinsJ D Chulay
Dec 1, 1990·The American Journal of Tropical Medicine and Hygiene·P H van VianenB Mons
Dec 1, 1986·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J D Hare
Nov 1, 1987·Cytometry·M T MaklerD Recktenwald
Nov 1, 1983·Cytometry·J W JacobbergerJ D Hare
Jan 1, 1983·Transactions of the Royal Society of Tropical Medicine and Hygiene·S ThaithongM Chutmongkonkul
May 1, 1980·The American Journal of Tropical Medicine and Hygiene·P Nguyen-Dinh, W Trager
Nov 1, 1980·The American Journal of Tropical Medicine and Hygiene·G V BrownR J Howard
Jan 1, 1980·Transactions of the Royal Society of Tropical Medicine and Hygiene·L Yisunsri, K Rieckmann

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Citations

Jan 21, 2000·Phytotherapy Research : PTR·F Traore-KeitaP Timon-David
Feb 27, 2007·European Journal of Medicinal Chemistry·Caritza LeónJosé N Domínguez
Aug 23, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Howard M Shapiro, Francis Mandy
Apr 26, 2005·Il Farmaco·José N DomínguezPhilip J Rosenthal
Apr 22, 2014·The Journal of Urology·Jacobien M KiefferNeil K Aaronson
Aug 9, 2019·Journal of Enzyme Inhibition and Medicinal Chemistry·D I UgwujaD I Ugwu
Jan 1, 1997·Journal of Clinical Microbiology·C WenischA Georgopoulos
May 13, 2005·Journal of Medicinal Chemistry·José N DomínguezPhilip J Rosenthal

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