Concanavalin A- and fetal-calf-serum-induced rounding and myosin light chain phosphorylation in cultured smooth muscle cells

Journal of Cellular Physiology
Y SasakiY Sasaki

Abstract

Rabbit aorta smooth muscle cells (SMC) in long-term culture retracted in less than 10 min in response to a sequential order of stimulations by concanavalin A (Con A) and fetal calf serum (FCS). With additional continuous stimulation by FCS, the SMC took on a circular shape and were anchored to the substrate by retraction fibrils. This rounding was observed only when the cells were sequentially stimulated by Con A and FCS. Depletion of intracellular Ca2+ stores by the addition of EGTA and Ca2+ ionophores inhibited the rounding. Transient phosphorylation of MLC20 was observed in the initial stage during the SMC rounding. The extent of monophosphorylated MLC20 increased for up to 5 min to a maximal value of 49%. The diphosphorylated form reached a maximal value of 29% within 2 min; then both forms of MLC20 decreased. The process of the SMC rounding was inhibited by antimycin A or cytochalasins, in a dose-dependent manner, findings which suggested a dependency on both metabolic energy and actin-containing microfilaments. The smooth-muscle-relaxing agent, HA1077, also inhibited the process of SMC rounding. These observations suggest that a cellular contractile process might be involved in rounding of SMC.

References

Jan 1, 1979·Physiological Reviews·J Chamley-CampbellR Ross
Jun 23, 1986·Biochimica Et Biophysica Acta·S CsabinaK Bárány
Nov 1, 1989·The American Journal of Physiology·B D GaylinnR A Murphy
Jan 1, 1989·Annual Review of Physiology·C M Hai, R A Murphy
Jan 1, 1985·Annual Review of Pharmacology and Toxicology·K E Kamm, J T Stull
Jun 30, 1988·Biochemical and Biophysical Research Communications·H YoshimiT Omae
Jul 1, 1987·The American Journal of Physiology·Y SasakiH Hidaka
Sep 1, 1985·The American Journal of Physiology·D R Hathaway, J R Haeberle
Jan 1, 1983·Differentiation; Research in Biological Diversity·S FlörkeW Engel
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·W G Kerrick, L Y Bourguignon
Nov 1, 1983·Analytical Biochemistry·D R Hathaway, J R Haeberle
Jan 1, 1983·Differentiation; Research in Biological Diversity·J ThybergM Sjölund

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