PMID: 9557883Apr 29, 1998Paper

Concanavalin A binding and endoglycosidase D resistance of beta1,2-xylosylated and alpha1,3-fucosylated plant and insect oligosaccharides

Glycoconjugate Journal
I B Wilson, Friedrich Altmann

Abstract

The binding to concanavalin A (Con A) by pyridylaminated oligosaccharides derived from bromelain (Manalpha1,6(Xylbeta1,2) Manbeta1, 4GlcNAcbeta1,4(Fucalpha1,3)GlcNAc), horseradish peroxidase (Manalpha1,6(Manalpha1,3) (Xylbeta1,2)Manbeta1, 4GlcNAcbeta1,4(Fucalpha1,3) GlcNAc), bee venom phospholipase A2 (Manalpha1,6Manbeta1,4GlcNAcbeta1,4GlcNAc and Manalpha1,6(Manalpha1,3)Manbeta1,4GlcNAcbeta1,4 (Fucalpha1,3)GlcNAc) and zucchini ascorbate oxidase (Manalpha1,6(Manalpha1,3) (Xylbeta1,2)Manbeta1,4 GlcNAcbeta1,4GlcNAc) was compared to the binding by Man3GlcNAc2, Man5GlcNAc2 and the asialo-triantennary complex oligosaccharide from bovine fetuin. While the fetuin oligosaccharide did not bind, bromelain, zucchini, Man2GlcNAc2 and horseradish peroxidase were retarded (in that order). The alpha1,3-fucosylated phospholipase, Man3GlcNAc2 and Man5GlcNAc2 structures were eluted with 15 mM alpha-methylmannoside. It is concluded that core alpha1,3-fucosylation has little or no effect on ConA binding while xylosylation decreases affinity for ConA. In a parallel study comparing the endoglycosidase D (Endo D) sensitivities of Man3GlcNAc2, IgG-derived GlcNAcbeta1, 2Manalpha1,6(GlcNAcbeta1,2Manalpha1,3)Manbeta1,+ ++4GlcNAcbeta1,4(Fucalpha1,6)GlcNAc,...Continue Reading

Citations

Jun 21, 2006·Biotechnology Letters·Pawan Gupta, M Saleemuddin
Sep 29, 2011·Plant Biotechnology Journal·Hélène ManduzioVéronique Gomord
Aug 29, 2003·The Plant Journal : for Cell and Molecular Biology·S PagnyV Gomord
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·A A McCormickR Levy

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