Concanavalin A distorts the beta-GlcNAc-(1-->2)-Man linkage of beta-GlcNAc-(1-->2)-alpha-Man-(1-->3)-[beta-GlcNAc-(1-->2)-alpha-Man- (1-->6)]-Man upon binding

Glycobiology
D N Moothoo, J H Naismith

Abstract

Carbohydrate recognition by proteins is a key event in many biological processes. Concanavalin A is known to specifically recognize the pentasaccharide core (beta-GlcNAc-(1-->2)-alpha- Man-(1-->3)-[beta-GlcNAc-(1-->2)-alpha-Man-(1-->6)]-Man) of N-linked oligosaccharides with a Ka of 1.41 x 10(6 )M-1. We have determined the structure of concanavalin A bound to beta-GlcNAc-(1-->2)-alpha-Man-(1-->3)-[beta-GlcNAc-(1-->2)-alpha-Man- (1-->6)]-Man to 2.7A. In six of eight subunits there is clear density for all five sugar residues and a well ordered binding site. The pentasaccharide adopts the same conformation in all eight subunits. The binding site is a continuous extended cleft on the surface of the protein. Van der Waals interactions and hydrogen bonds anchor the carbohydrate to the protein. Both GlcNAc residues contact the protein. The GlcNAc on the 1-->6 arm of the pentasaccharide makes particularly extensive contacts and including two hydrogen bonds. The binding site of the 1-->3 arm GlcNAc is much less extensive. Oligosaccharide recognition by Con A occurs through specific protein carbohydrate interactions and does not require recruitment of adventitious water molecules. The beta-GlcNAc-(1-->2)-Man glycosidic linkage PSI torsi...Continue Reading

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J K ScottI J Goldstein
May 5, 1992·Journal of Molecular Biology·S L Mowbray, L B Cole
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Nov 21, 1972·Biochemistry·K H Ibsen, P Trippet
Aug 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·M I Page, W P Jencks
Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·A ZdanovM Cygler
Sep 1, 1994·Nature Structural Biology·P E SteinR J Read
Jan 1, 1995·Annual Review of Biophysics and Biomolecular Structure·J M Rini
Feb 1, 1994·Protein Science : a Publication of the Protein Society·E A MerrittW G Hol
Jan 12, 1996·The Journal of Biological Chemistry·J H Naismith, R A Field
Mar 19, 1996·Biochemistry·R V WeathermanE J Toone
Jul 26, 1996·The Journal of Biological Chemistry·A SuroliaF P Schwarz
Jun 1, 1996·Protein Science : a Publication of the Protein Society·F van den AkkerW G Hol
Dec 1, 1996·Veterinary Immunology and Immunopathology·Y Ni, I Tizard
Mar 1, 1997·The Journal of Clinical Investigation·J B Lowe, P A Ward
Nov 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·E A Merritt, M E Murphy
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Jan 1, 1996·Acta Crystallographica. Section D, Biological Crystallography·S J HarropJ Yariv
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·W P Jencks

❮ Previous
Next ❯

Citations

Jan 11, 2007·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yoshinori Iiguni, Hitoshi Watarai
Jul 8, 2005·Proteins·Alain Laederach, Peter J Reilly
Sep 12, 2008·Journal of Computational Chemistry·Amanda M SalisburgKarl N Kirschner
Jan 12, 2011·Expert Opinion on Biological Therapy·Mark AgostinoElizabeth Yuriev
Sep 10, 2003·Journal of Computational Chemistry·Alain Laederach, Peter J Reilly
Jun 1, 2001·The Journal of Biological Chemistry·G Cunto-AmestyT Kieber-Emmons
Jun 8, 2013·Chemical Communications : Chem Comm·Jian-Tao ZhangSanford A Asher
Jun 14, 2012·The Journal of Biological Chemistry·Raquel Guimarães BenevidesAnne Imberty
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·C A BushA Imberty
Jul 11, 2018·International Journal of Molecular Sciences·Uthailak NaphatsamonKazuhito Fujiyama
Apr 13, 2019·Macromolecular Bioscience·Alberto Camaleño de la CalleStephan Schmidt
Jan 13, 2019·International Journal of Molecular Sciences·Annick BarrePierre Rougé
Oct 13, 2020·American Journal of Medical Genetics. Part a·Sheri A PoskanzerChristina Lam
Dec 2, 2004·Progress in Biophysics and Molecular Biology·Istvan Botos, Alexander Wlodawer
Dec 5, 2008·Journal of the American Chemical Society·Renuka KadirvelrajRobert J Woods
Jul 17, 2001·Journal of Molecular Biology·D A SandersJ H Naismith
Mar 5, 1999·Journal of Molecular Biology·A Kasuya, J M Thornton
Oct 6, 1999·Current Opinion in Structural Biology·J BouckaertR Loris
May 21, 2019·International Journal of Biological Macromolecules·Benildo Sousa CavadaKyria Santiago Nascimento
Nov 14, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Eric OgharandukunPreethi L Chandran
Sep 11, 2019·Organic Letters·Anne BrinkøHenrik H Jensen
Nov 23, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Hara JangSeung Jae Lee

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.