PMID: 6160612Jan 1, 1980Paper

Concanavalin A-mediated in vitro activation of a secondary cytotoxic T-cell response in virus-primed splenocytes

Scandinavian Journal of Immunology
A R Thomsen, B L Jensen

Abstract

In a recent report it was shown that what appeared to be secondary cytotoxic T cells could be obtained from lymphocytic choriomeningitis virus (LCMV)-primed splenocytes after stimulation in vitro with the non-specific T cell mitogen concanavalin A (Con A). The present experiments attempt to characterize further these effector cells and, in particular, to establish whether the Con A-activated cytotoxic effectors are qualitatively different from the secondary cytotoxic T cells induced by restimulation with the homologous antigen. It was found that: (1) in vitro activation with Con A could be obtained with populations harvested between 13 days (the earliest tested) and at least 300 days after priming; (2) cytotoxicity was independent of the presence of carried-over Con A in the cytotoxicity assay; (3) cytotoxicity was dependent on close association between activated T cells and target cells, since no evidence was found to indicate a role for other cell types or soluble (cytotoxic or arming) factors; (4) cytotoxicity was specific with regard to both virus and 'self'. By comparison with previous data on LCMV-induced cytotoxic T cells, it is concluded that Con A induces the generation of cytotoxic T cells from LCMV-primed splenocytes...Continue Reading

References

Jan 1, 1977·Immunological Reviews·I F McKenzieR V Blanden
Jan 1, 1977·Immunological Reviews·F H BachM L Bach
Mar 1, 1978·The Journal of Experimental Medicine·R M ZinkernagelJ Klein
Jan 1, 1979·The Journal of Experimental Medicine·P E BakerK A Smith
May 1, 1974·The Journal of Experimental Medicine·M VolkertK Bro-Jorgensen
Oct 1, 1973·European Journal of Immunology·M H JuliusL A Herzenberg
Jun 1, 1973·The Journal of Experimental Medicine·O Marker, M Volkert
Oct 1, 1970·Experimental Cell Research·A E Powell, M A Leon
Jan 1, 1964·Acta Pathologica Et Microbiologica Scandinavica·M VOLKERTC PFAU

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