Concave microwell array-mediated three-dimensional tumor model for screening anticancer drug-loaded nanoparticles

Nanomedicine : Nanotechnology, Biology, and Medicine
Ah Ran KangSang-Hoon Lee

Abstract

We investigated the effect of anticancer drug-loaded functional polymeric nanoparticles on drug resistance of three-dimensional (3D) breast tumor spheroids. 3D tumor models were built using concave microwells with different diameters (300-700μm) and nanoparticles were prepared using thermo-responsive poly(N-isopropylacrylamide) (PNIPAM)-co-acrylic acid (AA). Upon culturing with doxorubicin-loaded PNIPAM-co-AA nanoparticles for 96hours, the smallest tumor spheroids were extensively disrupted, resulting in a reduction in spheroid diameter. In contrast, the sizes of the largest tumor spheroids were not changed. Scanning electron microscopy revealed that the circular shape of 3D spheroids treated with doxorubicin-loaded PNIPAM-co-AA nanoparticles had collapsed severely. Cell viability assays also demonstrated that the largest tumor spheroids cultured with doxorubicin-loaded PNIPAM-co-AA nanoparticles were highly resistant to the anticancer drug. We confirmed that tight cell-cell contacts within largest tumor spheroids significantly improved the anticancer drug resistance. Therefore, this uniform-sized 3D breast tumor model could be a potentially powerful tool for anticancer drug screening applications. The battle against cancer is ...Continue Reading

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Citations

May 5, 2018·American Journal of Reproductive Immunology : AJRI·Wendy FitzgeraldLeonid Margolis
Mar 3, 2020·Nanoscale Horizons : the Home for Rapid Reports of Exceptional Significance in Nanoscience and Nanotechnolgy·Emilie DarriguesAnaliz Rodriguez
Mar 16, 2021·Acta Biomaterialia·Mackenzie L Grubb, Steven R Caliari
Mar 9, 2021·Frontiers in Bioengineering and Biotechnology·Pauline MaurySandrine Lacombe

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