Concentrated Conditioned Media from Adipose Tissue Derived Mesenchymal Stem Cells Mitigates Visual Deficits and Retinal Inflammation Following Mild Traumatic Brain Injury

International Journal of Molecular Sciences
Kumar Abhiram JhaRajashekhar Gangaraju

Abstract

Blast concussions are a common injury sustained in military combat today. Inflammation due to microglial polarization can drive the development of visual defects following blast injuries. In this study, we assessed whether anti-inflammatory factors released by the mesenchymal stem cells derived from adipose tissue (adipose stem cells, ASC) can limit retinal tissue damage and improve visual function in a mouse model of visual deficits following mild traumatic brain injury. We show that intravitreal injection of 1 μL of ASC concentrated conditioned medium from cells pre-stimulated with inflammatory cytokines (ASC-CCM) mitigates loss of visual acuity and contrast sensitivity four weeks post blast injury. Moreover, blast mice showed increased retinal expression of genes associated with microglial activation and inflammation by molecular analyses, retinal glial fibrillary acidic protein (GFAP) immunoreactivity, and increased loss of ganglion cells. Interestingly, blast mice that received ASC-CCM improved in all parameters above. In vitro, ASC-CCM not only suppressed microglial activation but also protected against Tumor necrosis alpha (TNFα) induced endothelial permeability as measured by transendothelial electrical resistance. Bioc...Continue Reading

References

Nov 24, 2004·Investigative Ophthalmology & Visual Science·Glen T PruskyRobert M Douglas
Sep 13, 2005·Experimental Eye Research·Ianors IandievThomas Pannicke
May 3, 2008·Investigative Ophthalmology & Visual Science·Ianors IandievAndreas Bringmann
Dec 2, 2008·Ophthalmology·Eric D WeichelAndrew S Eiseman
Mar 24, 2009·Journal of Neurosurgery·Matthew T HartingCharles S Cox
Jun 13, 2009·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Karen D BrahmGregory L Goodrich
Sep 24, 2009·Experimental Hematology·Jaehyup Kim, Peiman Hematti
Nov 26, 2009·Investigative Ophthalmology & Visual Science·Thomas V JohnsonKeith R Martin
Dec 15, 2010·Stem Cell Research & Therapy·Elise DantumaKiminobu Sugaya
Feb 18, 2011·Seminars in Immunopathology·Kenneth A Newell
Apr 6, 2012·Journal of the Neurological Sciences·Jin Young ChungSeung Myung Moon
Apr 17, 2012·Experimental Eye Research·Jessica Hines-BeardTonia S Rex
Aug 1, 2012·Rehabilitation Research and Practice·Christopher S Sahler, Brian D Greenwald
Aug 17, 2012·Journal of Cardiovascular Pharmacology and Therapeutics·Shimrit Adutler-LieberJonathan Leor
Oct 2, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Lavinia IonescuBernard Thébaud
Oct 16, 2012·Journal of Neuroinflammation·Mahasweta DasShyam S Mohapatra
Jan 19, 2013·Nature Reviews. Neuroscience·Ye XiongMichael Chopp
Jan 31, 2013·Journal of Neurotrauma·J Regino Perez-PoloClaire E Hulsebosch
Mar 29, 2014·Experimental Eye Research·Gangaraju RajashekharSangly P Srinivas
May 6, 2014·Frontiers in Endocrinology·Gangaraju Rajashekhar
May 13, 2014·Cell Transplantation·Tzu-Min ChanHorng-Jyh Harn
Jun 4, 2014·Journal of Neuroinflammation·Jonathan D CherryM Kerry O'Banion
Jun 27, 2014·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Elisa R ZanierMaria-Grazia De Simoni
Jul 6, 2014·Investigative Ophthalmology & Visual Science·Courtney Bricker-AnthonyTonia S Rex

❮ Previous
Next ❯

Citations

Apr 4, 2019·Molecular Neurobiology·Eliana Baez-JuradoGeorge E Barreto
Sep 19, 2020·International Journal of Molecular Sciences·Santiago RouraFrancesc E Borràs
Oct 8, 2020·Stem Cells Translational Medicine·Vanessa CastelliAnnamaria Cimini
Sep 11, 2020·Experimental Eye Research·Leandro C Teixeira-PinheiroMarcelo F Santiago
Apr 28, 2021·Stem Cell Reviews and Reports·Maria Luz Alonso-AlonsoYolanda Diebold
May 4, 2021·Frontiers in Molecular Neuroscience·Lucy P EvansAlexander G Bassuk

❮ Previous
Next ❯

Methods Mentioned

BETA
protein assay
PCR
Assay

Software Mentioned

GraphPad Prism
OptoMotry
limma
Labview

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.