Concentrated protein body product derived from rice endosperm as an oral tolerogen for allergen-specific immunotherapy--a new mucosal vaccine formulation against Japanese cedar pollen allergy

PloS One
Yuhya WakasaFumio Takaiwa

Abstract

The endoplasmic reticulum-derived type-I protein body (PB-I) from rice endosperm cells is an ideal candidate formulation for the oral delivery of bioencapsulated peptides as tolerogens for allergen-specific immunotherapy. In the present study, PBs containing the deconstructed Japanese cedar pollen allergens Cryptomeria japonica 1 (Cry j 1) and Cry j 2 were concentrated by treatment with thermostable α-amylase at 90°C to remove the starch from milled rice powder, which resulted in a 12.5-fold reduction of dry weight compared to the starting material. The modified Cry j 1 and Cry j 2 antigens in this concentrated PB product were more resistant to enzymatic digestion than those in the milled seed powder despite the absence of intact cell wall and starch, and remained stable for at least 10 months at room temperature without detectable loss or degradation. The high resistance of these allergens could be attributed to changes in protein physicochemical properties induced by the high temperature concentration process, as suggested by the decreased solubility of the antigens and seed proteins in PBs in step-wise-extraction experiments. Confocal microscopy showed that the morphology of antigen-containing PB-Is was preserved in the conc...Continue Reading

References

Jun 3, 2004·Nature Reviews. Immunology·Lloyd Mayer, Ling Shao
Apr 16, 2005·Current Opinion in Biotechnology·Eva StogerPaul Christou
Jan 25, 2006·Methods : a Companion to Methods in Enzymology·Stephen J Streatfield
Dec 19, 2006·Plant Biotechnology Journal·Hidenori TakagiFumio Takaiwa
Dec 21, 2006·Journal of Agricultural and Food Chemistry·Hidenori TakagiFumio Takaiwa
Jun 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Tomonori NochiHiroshi Kiyono
Oct 7, 2008·Trends in Immunology·Noriko M Tsuji, Akemi Kosaka
May 23, 2009·Biotechnology Advances·On Sun Lau, Samuel S M Sun
Mar 2, 2011·Human Vaccines·Fumio Takaiwa
Aug 11, 2011·Advanced Drug Delivery Reviews·Jun KunisawaHiroshi Kiyono
Feb 10, 2012·Mucosal Immunology·O Pabst, A M Mowat
Feb 12, 2013·Current Pharmaceutical Design·Maite SabalzaTeresa Capell
Oct 5, 2013·Biotechnology Journal·Yuhya Wakasa, Fumio Takaiwa
Jul 1, 1988·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·T KumamaruK Tanaka
Jan 15, 2014·Plant Cell Reports·Jiquan OuDaichnag Yang

❮ Previous
Next ❯

Citations

Dec 29, 2015·Trends in Biotechnology·Sergio Rosales-MendozaBeatriz Meza
Jun 24, 2015·Plant Biotechnology Journal·Fumio TakaiwaTakachika Hiroi
Feb 19, 2016·Expert Review of Vaccines·Sergio Rosales-MendozaRalf Reski
Oct 28, 2016·GM Crops & Food
Apr 3, 2016·British Journal of Clinical Pharmacology·Matilde MerlinLinda Avesani
Aug 31, 2017·Human Vaccines & Immunotherapeutics·Yan SuTeri Heiland
Aug 17, 2021·Biotechnology and Bioprocess Engineering : BBE·Siddhesh B GhagVishwas A Bapat

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
ELISA
confocal microscopy
Confocal

Related Concepts

Related Feeds

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Antiparasitics

Antiparasitics are medications which are indicated for the treatment of parasitic diseases. Discover the latest research on antiparasitics here.

Allergies & Environmental Factors

Environmental factors are strongly associated with the prevalence of allergies and are an increasing health concern worldwide. Discover the latest research on Allergies and Environmental Factors here.

Anaphylaxis

Anaphylaxis is a serious allergic reaction that is rapid in onset and may cause death.