Concentrated Solutions of Single-Chain Nanoparticles: A Simple Model for Intrinsically Disordered Proteins under Crowding Conditions

The Journal of Physical Chemistry Letters
Angel J MorenoJuan Colmenero

Abstract

By means of large-scale computer simulations and small-angle neutron scattering (SANS), we investigate solutions of single-chain nanoparticles (SCNPs), covering the whole concentration range from infinite dilution to melt density. The analysis of the conformational properties of the SCNPs reveals that these synthetic nano-objects share basic ingredients with intrinsically disordered proteins (IDPs), as topological polydispersity, generally sparse conformations, and locally compact domains. We investigate the role of the architecture of the SCNPs in their collapse behavior under macromolecular crowding. Unlike in the case of linear macromolecules, which experience the usual transition from self-avoiding to Gaussian random-walk conformations, crowding leads to collapsed conformations of SCNPs resembling those of crumpled globules. This behavior is already found at volume fractions (about 30%) that are characteristic of crowding in cellular environments. The simulation results are confirmed by the SANS experiments. Our results for SCNPs--a model system free of specific interactions--propose a general scenario for the effect of steric crowding on IDPs: collapse from sparse conformations at high dilution to crumpled globular conform...Continue Reading

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Citations

Jul 21, 2017·Physical Chemistry Chemical Physics : PCCP·Emanuela BianchiPeter D J van Oostrum
Dec 6, 2017·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Angel J MorenoJosé A Pomposo
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Sep 13, 2019·Soft Matter·Dieter Richter, Margarita Kruteva
May 12, 2016·Macromolecular Rapid Communications·Senda BasasoroJosé A Pomposo
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Mar 4, 2020·Polymers·Mariarita PaciollaAngel J Moreno
Feb 26, 2019·Macromolecular Rapid Communications·Jon Rubio-CervillaJosé A Pomposo
Dec 8, 2020·Macromolecular Rapid Communications·Daniel E Martínez-TongEster Verde-Sesto
Jan 20, 2021·Soft Matter·Maud Formanek, Angel J Moreno
Aug 9, 2021·The Journal of Chemical Physics·Xiang-Meng JiaZhong-Yuan Lu
Aug 21, 2018·The Journal of Organic Chemistry·Donatien de RochambeauHanadi F Sleiman

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