Concentration and pH dependence of skeletal muscle chloride channel ClC-1

The Journal of Physiology
Grigori Y RychkovAllan H Bretag

Abstract

1. The influence of Cl- concentration and pH on gating of the skeletal muscle Cl- channel, ClC-1, has been assessed using the voltage-clamp technique and the Sf-9 insect cell and Xenopus oocyte expression systems. 2. Hyperpolarization induces deactivating inward currents comprising a steady-state component and two exponentially decaying components, of which the faster is weakly voltage dependent and the slower strongly voltage dependent. 3. Open probability (Po) and kinetics depend on external but not internal Cl- concentration. 4. A point mutation, K585E, in human ClC-1, equivalent to a previously described mutation in the Torpedo electroplaque chloride channel, ClC-0, alters the I-V relationship and kinetics, but retains external Cl- dependence. 5. When external pH is reduced, the deactivating inward currents of ClC-1 are diminished without change in time constants while the steady-state component is enhanced. 6. In contrast, reduced internal pH slows deactivating current kinetics as its most immediately obvious action and the Po curve is shifted in the hyperpolarizing direction. Addition of internal benzoate at low internal pH counteracts both these effects. 7. A current activated by hyperpolarization can be revealed at an e...Continue Reading

Citations

Mar 19, 2014·Pflügers Archiv : European journal of physiology·Gabriel StöltingChristoph Fahlke
Nov 30, 2005·Cellular and Molecular Life Sciences : CMLS·M SuzukiT Iwamoto
Dec 22, 2011·Journal of Biomedicine & Biotechnology·Chih-Yung Tang, Tsung-Yu Chen
Apr 27, 1999·British Journal of Pharmacology·E C AromatarisM L Roberts
Mar 1, 2008·Critical Reviews in Biochemistry and Molecular Biology·Thomas J Jentsch
Mar 2, 1999·The Journal of Physiology·M PuschT J Jentsch
Oct 9, 2013·The Journal of Physiology·Silvia De StefanoGiovanni Zifarelli
Nov 29, 2002·The Journal of Physiology·Aisling RyanChristoph Fahlke
Jul 9, 2005·The Journal of Physiology·Michael D DuffieldMichael L Roberts
May 30, 2012·The Journal of Physiology·Sebastian WeinbergerMartin Fischer
Apr 28, 2010·The Journal of Physiology·Andrew J Smith, Jonathan D Lippiat
Sep 20, 2008·The Journal of Physiology·Jennie Garcia-OlivaresChristoph Fahlke
May 26, 2010·The Journal of Physiology·Jorge E Sánchez-RodríguezJorge Arreola
Dec 17, 2015·The Journal of General Physiology·José J De Jesús-PérezJorge Arreola
Sep 23, 2014·Journal of Theoretical Biology·Weifan Liu, Sarah D Olson
Aug 17, 2001·The Journal of Physiology·B BennettsG Y Rychkov
Sep 2, 2004·Proteins·Jian YinThomas L Beck
Jan 16, 2015·The Journal of Physiology·Thomas J Jentsch
Mar 30, 2011·The Journal of General Physiology·Pang-Yen TsengTsung-Yu Chen
Jan 14, 2004·The Journal of General Physiology·Alessio AccardiChristopher Miller
Aug 30, 2000·The Journal of General Physiology·A Accardi, M Pusch
Feb 3, 2005·The Journal of General Physiology·Thomas H PedersenOle B Nielsen
Dec 1, 2000·The Journal of General Physiology·Z Qu, H C Hartzell
Jun 29, 2001·The Journal of General Physiology·M F Chen, T Y Chen
Dec 15, 2010·The Journal of General Physiology·Marino DiFrancoJulio L Vergara
Dec 15, 2010·The Journal of General Physiology·Christoph Fahlke
Nov 17, 2010·The Journal of General Physiology·John D LueckRobert T Dirksen
Nov 17, 2010·The Journal of General Physiology·Giovanni Zifarelli, Michael Pusch
Jan 30, 2008·The Journal of General Physiology·Alessio Accardi
Jan 30, 2008·The Journal of General Physiology·Giovanni Zifarelli, Michael Pusch
Nov 16, 2005·The Journal of General Physiology·Jose Antonio de SantiagoJorge Arreola
Dec 29, 2005·The Journal of General Physiology·Sonia TraversoMichael Pusch
Sep 2, 2010·The Journal of General Physiology·Antonella GradognaMichael Pusch
Feb 5, 2003·The Journal of General Physiology·Michael DuffieldMichael Roberts
Aug 1, 1997·The Journal of General Physiology·U LudewigM Pusch
Sep 1, 1997·The Journal of General Physiology·T VoetsB Nilius
Jun 20, 1998·The Journal of General Physiology·G Y RychkovA H Bretag
Apr 9, 2005·IEEE Transactions on Nanobioscience·Michael Pusch, Thomas J Jentsch

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