Concentration and time effects of dextran exposure on endothelial cell viability, attachment, and inflammatory marker expression in vitro

Annals of Biomedical Engineering
Leonie RouleauRichard L Leask

Abstract

Dextran is commonly used to alter growth medium rheological properties for in vitro flow experiments in order to match physiological parameters. Despite its acceptance in literature, few studies have examined dextran effects on cells. In this study, we investigated changes in endothelial cell function due to dextran, under static and flow conditions, in a concentration and time-dependent manner. Dextran increased endothelial cell viability, decreased their ability to attach to culture plates and decreased leukocyte adhesion to endothelial cells. Under static conditions, dextran increased protein and mRNA expression of intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) in a concentration and time-dependent manner and caused the nuclear translocation of NF-kappaB. Steady laminar wall shear stress modulated the effects of dextran on ICAM-1, VCAM-1, and NF-kappaB expression in straight/tubular in vitro models. When the expression was normalized to their respective time matched static dextran control, it did not affect the ability to detect changes caused by shear on the mRNA expression of ICAM-1 and VCAM-1. This study demonstrates that dextran can alter endothelial cell function and therefore,...Continue Reading

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Citations

Jul 31, 2010·Journal of Biomechanical Engineering·Leonie RouleauRichard L Leask
Apr 25, 2014·Physiological Reports·Syotaro ObiTakayuki Asahara
Jul 19, 2011·Biotechnology and Bioengineering·Amber L DoironKristina D Rinker
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May 6, 2014·Bioorganic & Medicinal Chemistry Letters·Aldo Moreno-UlloaIsrael Ramirez-Sanchez
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Dec 2, 2015·Cardiovascular Engineering and Technology·Hamed AvariKem A Rogers
Jul 24, 2015·Journal of Biomaterials Applications·Jun MaDonghui Zhu
May 13, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Erika M J SirenJayachandran N Kizhakkedathu

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