PMID: 9545559Apr 18, 1998Paper

Concentration-dependent differential effects of N-acetyl-L-cysteine on the expression of HSP70 and metallothionein genes induced by cadmium in human amniotic cells

Biochimica Et Biophysica Acta
T AbeK Higashi

Abstract

Cadmium induces the expression of the 70 kDa heat shock protein (HSP70) and metallothionein (MT), both of which are considered to be associated with intracellular glutathione (GSH) metabolism in the cellular protection mechanism against cadmium-induced cellular injury. We determined the effects of N-acetyl-L-cysteine (NAC), which increases the intracellular GSH levels, on the induction of HSP70 and MT gene expression in a cultured cell line of human amniotic cells (WISH) exposed to CdCl2. The mRNA level of MT-II, a major isoform of MT genes, was more prominently increased than that of HSP70 when WISH cells were exposed to CdCl2 (5-15 microM, for 6 h). The treatment of WISH cells with 1.5 and 30 mM NAC for 2 h increased the intracellular GSH levels by 1.4- and 3.1-fold, respectively. Pretreatment of cells with 30 mM NAC significantly reduced both HSP70 and MT-II mRNA levels in the cells exposed to 50 microM CdCl2. This concentration of NAC also efficiently suppressed the cadmium-induced lethality. On the contrary, pretreatment with 1.5 mM NAC suppressed only the induction of HSP70 gene expression in the 50 microM CdCl2-treated cells, and did not inhibit the metal toxicity. However, this low concentration of NAC efficiently suppr...Continue Reading

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Citations

Mar 4, 2000·Free Radical Biology & Medicine·R G Allen, M Tresini
Jan 26, 2016·International Journal of Molecular Sciences·Adolf Michael Sandbichler, Martina Höckner
Aug 20, 2014·BioMed Research International·Khalid MahmoodJamshaid Hussain
Jul 31, 2010·Birth Defects Research. Part A, Clinical and Molecular Teratology·Yolanda MacKinnon, Carolyn M Kapron
Dec 16, 2016·Oxidative Medicine and Cellular Longevity·Alexandra SakelliouAsimina Mitrakou
Apr 10, 2021·Environmental Science and Pollution Research International·Mohammad Ehsan Taghavizadeh YazdiSeyed Hadi Mousavi

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