Concentration-dependent inhibitory effects of apolipoprotein E on Alzheimer's beta-amyloid fibril formation in vitro

Biochemistry
H NaikiK Nakakuki

Abstract

Recently, many research groups have examined the effect of apolipoprotein E (apoE) on beta-amyloid fibril (betaAf) formation in vitro. However, their data were somewhat controversial and no exact kinetic assessment of the role of apoE has thus far been available. We examined the effect of human apoE on betaAf formation in vitro, starting with various concentrations of freshly prepared beta-amyloid(1-40) (beta1-40) and using fluorescence spectroscopy with thioflavine T. When 50 microM of beta1-40 was incubated with a 1:1000 to 1:100 molar ratio of apoE, a dose-dependent inhibitory effect of apoE was observed. Both the nucleation and extension phases of betaAf formation in vitro were inhibited by apoE. On the other hand, when 300 microM of beta1-40 was incubated with a 1:100 molar ratio of apoE, the inhibitory effect of apoE was completely abolished. We then focused our study on the kinetics of the inhibitory effect of apoE on the extension phase of betaAf formation in vitro, utilizing the recently established first-order kinetic model of betaAf extension in vitro [Naiki, H., & Nakakuki, K. (1996) Lab. Invest. 74, 374-383]. The mathematical treatment of the data suggests that apoE inhibits the extension of betaAf in vitro, by mak...Continue Reading

References

Feb 15, 1991·Clinica Chimica Acta; International Journal of Clinical Chemistry·J CarlssonD Seidel
Mar 1, 1995·The Biochemical Journal·E M CastanoB Frangione
Nov 3, 1994·Nature·T OkanoY Fukada

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Citations

Aug 11, 2000·Microscopy Research and Technique·L BaumC P Pang
Mar 15, 2011·Protein & Cell·Chai Lean TeohGeoffrey J Howlett
Apr 23, 2003·Biochemical and Biophysical Research Communications·Kazuhiro HasegawaHironobu Naiki
Oct 26, 2002·Biological Psychiatry·Kenjiro OnoHironobu Naiki
Jul 11, 2002·Free Radical Biology & Medicine·Miguel A PappollaLorenzo M Refolo
Aug 1, 1997·Current Opinion in Chemical Biology·P T Lansbury
Nov 14, 2002·Biochimica Et Biophysica Acta·Naoki TakahashiHironobu Naiki
Dec 7, 2007·Nature Structural & Molecular Biology·Sandra ChimonYoshitaka Ishii
Aug 10, 2000·Neuropathology : Official Journal of the Japanese Society of Neuropathology·M Yamada
Jun 18, 2002·Journal of Neurochemistry·Kenjiro OnoHironobu Naiki
Jan 11, 2011·The Journal of Biological Chemistry·Daisaku OzawaHironobu Naiki
Jul 7, 2012·The Journal of Biological Chemistry·Rosa CrespoPedro M Martins
Oct 27, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tadafumi HashimotoBradley T Hyman
Jan 29, 2013·Annual Review of Biochemistry·Amy R WyattMark R Wilson
Nov 4, 2015·Brain : a Journal of Neurology·Rajnish KumarTaher Darreh-Shori
Aug 1, 1998·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·D B Teplow
Jan 18, 2012·Journal of Molecular Biology·Amy R WyattMark R Wilson
Mar 24, 2009·Bone·Suguru YamamotoFumitake Gejyo
Mar 3, 2004·Journal of Neuroscience Research·Kenjiro OnoMasahito Yamada
Feb 26, 2008·Chembiochem : a European Journal of Chemical Biology·Haijia YuXiaogang Qu
Jul 16, 2013·Journal of Peptide Science : an Official Publication of the European Peptide Society·Md ShahnawazIl-Seon Park
Jan 10, 2012·Geriatrics & Gerontology International·Kenjiro Ono, Masahito Yamada
Nov 10, 2004·Journal of Molecular Biology·Tadato BanYuji Goto
Oct 20, 2006·Bioorganic & Medicinal Chemistry·Céline RivièreJean-Pierre Monti
Dec 14, 2005·Neurochemistry International·Kenjiro OnoMasahito Yamada
Oct 26, 2005·Neurobiology of Disease·Kenjiro OnoMasahito Yamada
Feb 25, 2014·Neuron·Takahisa KanekiyoGuojun Bu
Mar 8, 2011·The International Journal of Biochemistry & Cell Biology·Silvia BologninPaolo Zatta
Aug 23, 2017·Neurochemical Research·Kenjiro Ono
Jun 8, 2000·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·H LeVine

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