Concentration-dependent patterns of leucine incorporation by coastal picoplankton

Applied and Environmental Microbiology
Cecilia Alonso, Jakob Pernthaler

Abstract

Coastal pelagic environments are believed to feature concentration gradients of dissolved organic carbon at a microscale, and they are characterized by pronounced seasonal differences in substrate availability for the heterotrophic picoplankton. Microbial taxa that coexist in such habitats might thus differ in their ability to incorporate substrates at various concentrations. We investigated the incorporation patterns of leucine in four microbial lineages from the coastal North Sea at concentrations between 0.1 and 100 nM before and during a spring phytoplankton bloom. Community bulk incorporation rates and the fraction of leucine-incorporating cells in the different populations were analyzed. Significantly fewer bacterial cells incorporated the amino acid before (13 to 35%) than during (23 to 47%) the bloom at all but the highest concentration. The incorporation rate per active cell in the prebloom situation was constant above 0.1 nM added leucine, whereas it increased steeply with substrate concentration during the bloom. At both time points, a high proportion of members of the Roseobacter clade incorporated leucine at all concentrations (55 to 80% and 86 to 94%, respectively). In contrast, the fractions of leucine-incorporat...Continue Reading

References

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Jul 6, 2000·Applied and Environmental Microbiology·H EilersR Amann
Sep 30, 2000·Applied and Environmental Microbiology·J M GonzálezM A Moran
Jun 1, 2002·Applied and Environmental Microbiology·Annelie PernthalerRudolf Amann
Nov 7, 2003·Applied and Environmental Microbiology·David L KirchmanMatthew T Cottrell
Nov 6, 2004·Applied and Environmental Microbiology·Jarone PinhassiCèlia Marrasé
Apr 7, 2005·Applied and Environmental Microbiology·Cecilia Alonso, Jakob Pernthaler
May 5, 2005·Applied and Environmental Microbiology·Gerhard J HerndlJakob Pernthaler
May 17, 2005·Environmental Microbiology·Hans-Peter GrossartThorsten Brinkhoff
Jun 4, 2005·Applied and Environmental Microbiology·Rex R MalmstromDavid L Kirchman
Aug 9, 2005·Applied and Environmental Microbiology·Annelie Pernthaler, Jakob Pernthaler
Mar 1, 1988·Applied and Environmental Microbiology·Hans-Georg HoppeKlaus Gocke

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Citations

Jan 15, 2010·The ISME Journal·Ryan J NewtonMary Ann Moran
Sep 15, 2007·Nature Reviews. Microbiology·Farooq Azam, Francesca Malfatti
Oct 30, 2007·Applied and Environmental Microbiology·Hongyue DangGuiqiao Huang
Jan 31, 2012·Applied and Environmental Microbiology·Mrinalini P NikradD L Kirchman
Jan 8, 2016·FEMS Microbiology Ecology·Monica Ricão CanelhasStefan Bertilsson
May 21, 2009·Environmental Microbiology·Martha SchattenhoferJakob Pernthaler
Jul 20, 2007·Environmental Microbiology·Xiaozhen MouMary Ann Moran
May 16, 2008·Environmental Microbiology·Silke Langenheder, James I Prosser
Oct 4, 2006·Environmental Microbiology·Cecilia Alonso, Jakob Pernthaler
Jun 16, 2012·Environmental Microbiology·Laura Gómez-ConsarnauJarone Pinhassi
Jun 16, 2010·Environmental Microbiology·Krista LongneckerBenjamin A S Van Mooy
Nov 19, 2014·Environmental Microbiology Reports·María Teresa PérezRuben Sommaruga
Nov 19, 2011·FEMS Microbiology Reviews·Niculina MusatMarcel M M Kuypers
Mar 22, 2018·FEMS Microbiology Ecology·Xavier Mayali, Peter K Weber
Dec 20, 2017·Proceedings of the National Academy of Sciences of the United States of America·Kristin BergauerGerhard J Herndl
Oct 8, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Marta Sebastián, Josep M Gasol
Feb 2, 2019·Frontiers in Microbiology·Carina BunseJarone Pinhassi
Jun 11, 2020·FEMS Microbiology Ecology·Winifred M JohnsonHeidi Smith

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