Concentration Monitoring of Plasma Ribavirin: Validation of a Liquid Chromatography-Mass Spectrometric Method and Clinical Sample Collection

Therapeutic Drug Monitoring
Nigel W BrownJohn M Tredger

Abstract

A liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed for routine measurement of ribavirin concentrations in EDTA-anticoagulated plasma. After protein precipitation, we used a bridged ethylene hybrid (hydrophilic interaction) chromatography column, 0.1 mmol/L ammonium formate pH 3.0, and a gradient of 85%-96% acetonitrile to achieve baseline separation of ribavirin from isobaric uridine. Quantitation was assured using both primary (m/z 245.3 > 113.0) and secondary transitions (m/z 245.3 > 96.0) of the protonated species. Chromatographic separation and column washing also negated interference from major phospholipid species. There was a linear relationship between concentration and response to 10 mg/L, with a minimum detectable level and a minimum level of quantitation both of 0.1 mg/L. Imprecision within the assay was <10% at 0.1 mg/L and <6% between assays for concentrations >0.4 mg/L. Bias was <4%. In clinical samples (n = 12), there was no difference in ribavirin concentrations obtained by an established liquid chromatographic assay with ultraviolet detection. Ribavirin concentrations were stable in plasma stored at room temperature for 3 days but then decreased significantly on day 7. Plasma conc...Continue Reading

References

Jan 1, 1990·The International Journal of Biochemistry·T Page, J D Connor
Nov 9, 1994·Molecular and Cellular Biochemistry·T W Traut
May 3, 2000·British Journal of Clinical Pharmacology·P GlueM Salfi
May 1, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Johnson Y N LauZhi Hong
Dec 25, 2002·Antimicrobial Agents and Chemotherapy·Sylvie LarratClaude Souvignet
Apr 19, 2003·British Journal of Clinical Pharmacology·Akihito TsubotaHiromitsu Kumada
Dec 19, 2003·Journal of Clinical Pharmacology·UNKNOWN Global Burden Of Hepatitis C Working Group
Feb 7, 2004·Clinical Chemistry·Kristian Linnet, Marina Kondratovich
Mar 16, 2005·Clinical Biochemistry·Mark M KushnirFrancis M Urry
Aug 10, 2005·Biomedical Chromatography : BMC·Francis Beaudry, Pascal Vachon
Nov 16, 2005·Reviews in Medical Virology·Jason D Graci, Craig E Cameron
Feb 25, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·James L LittleCharles M Buchanan
Sep 12, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wenkui LiFrancis L S Tse
Sep 15, 2006·Journal of Separation Science·Petrus Hemström, Knut Irgum
Dec 16, 2006·Current Medicinal Chemistry·Stefan RussmannGiuseppe Palasciano
Jun 19, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Arianna LoregianGiorgio Palù
Jul 14, 2007·Journal of Pharmaceutical and Biomedical Analysis·M Espinosa BoschC Bosch Ojeda
Apr 26, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Véronique Loustaud-RattiElisabeth Vidal
Jul 25, 2008·Biomedical Chromatography : BMC·Zhi-Ling ZhouShu-Guang Lin
Oct 22, 2008·The Journal of Antimicrobial Chemotherapy·Judit MorelloVincent Soriano
Jan 24, 2009·The Journal of Pharmacology and Experimental Therapeutics·Christopher J EndresJashvant D Unadkat
Apr 7, 2009·Expert Review of Anti-infective Therapy·Véronique Loustaud-RattiSophie Alain
May 5, 2009·Therapeutic Drug Monitoring·Francois-Ludovic SauvagePierre Marquet
Feb 27, 2010·Journal of Hepatology·Yukina FukuchiKan Chiba
Dec 15, 2010·Journal of Pharmaceutical and Biomedical Analysis·Elisa ZironiGiampiero Pagliuca
Jul 13, 2011·Liver International : Official Journal of the International Association for the Study of the Liver·Chrisoula PipiliEvangelos Cholongitas
Jun 12, 2013·Journal of Chromatographic Science·Rim Said HaggagOla Ahmed El Rouby
Aug 15, 2013·Therapeutic Drug Monitoring·Sandra BodeauEtienne Brochot
Apr 23, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Mario U Mondelli

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