Concentration of cell-free DNA in different tumor types.

Expert Review of Molecular Diagnostics
O E BryzgunovaP P Laktionov

Abstract

Introduction: Cell-free DNA (cfDNA) circulates in the blood for a long time. The levels of cfDNA in the blood are assayed in cancer diagnostics because they are closely related to the tumor burden of patients.Areas covered: cfDNA escapes the action of DNA-hydrolyzing enzymes, being a part of supramolecular complexes or interacting with the plasma membrane of blood cells. cfDNA has heterogeneous size and composition, which impose various restrictions on both isolation methods and subsequent analysis. cfDNA concentration and structural changes with the development of diseases highlight the high potential of cfDNA as a diagnostic and prognostic marker. The concentration of cfDNA released in the blood by tumor cells determines the specificity of such diagnostics and the required blood volume. The present review aimed to synthesize the available data on cfDNA concentration in the cancer patient's blood as well as pre-analytical, analytical, and biological factors, which interfere with cfDNA concentration.Expert opinion: The concentration of cfDNA and tumor cell DNA (ctDNA), and the over-presentation of DNA loci in cfDNA must be considered when looking for tumor markers. Some inconsistent data on cfDNA concentrations (like those obta...Continue Reading

References

May 1, 1977·The American Journal of Medicine·C R Steinman, A Ackad
Nov 5, 1992·Journal of Immunological Methods·U E GibsonD V Sinicropi
May 1, 1992·Science·A M ShusterA G Gabibov
Mar 1, 1990·Journal of Clinical Microbiology·R BoomJ van der Noordaa
Jun 1, 1989·Arthritis and Rheumatism·J Z Li, C R Steinman
Oct 14, 1987·Biochemical and Biophysical Research Communications·M B Pepys, P J Butler
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·E Lazarides, U Lindberg
Jan 1, 1973·Arthritis and Rheumatism·G L Davis, J S Davis
Dec 1, 1980·The Journal of Clinical Investigation·L Raptis, H A Menard
Jan 1, 1994·Advances in Experimental Medicine and Biology·X Y Zhao, T W Hutchens
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·J G HermanS B Baylin
Mar 27, 2001·Methods in Molecular Biology·C Q PanR A Lazarus
May 29, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Tsu-Lan WuJames T Wu
Aug 3, 2002·Oncogene·Egor Prokhortchouk, Brian Hendrich
Apr 26, 2003·Analytical Chemistry·Michael C BreadmoreJames P Landers
Apr 10, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Thian-Sze WongAnthony Po-Wing Yuen
Jul 15, 2004·Annals of the New York Academy of Sciences·Bret TabackDave S B Hoon
Jul 15, 2004·Annals of the New York Academy of Sciences·Darrell AllenR Swaminathan
Jul 15, 2004·Annals of the New York Academy of Sciences·Pavel P LaktionovValentin V Vlassov
Nov 25, 2004·Nucleosides, Nucleotides & Nucleic Acids·Pavel P LaktionovValentin V Vlassov
Jan 20, 2005·Journal of the National Cancer Institute·Long-Cheng LiRajvir Dahiya
Mar 5, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jane L BoddyJim S Wainscoat
Jun 25, 2005·Clinical Chemistry·Svetlana N TamkovichPavel P Laktionov
Jul 20, 2005·Bulletin of Experimental Biology and Medicine·S N TamkovichV V Vlasov
Jul 26, 2005·Clinical Chemistry·Stefan HoldenriederY M Dennis Lo
Dec 22, 2005·Journal of the National Cancer Institute·Gabriella SozziUgo Pastorino
Sep 12, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Naoyuki UmetaniDave S B Hoon
Nov 3, 2006·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Carolina J JorgezFarideh Z Bischoff
Nov 17, 2006·Annals of the New York Academy of Sciences·Svetlana N TamkovichPavel P Laktionov
Dec 14, 2006·Clinical Chemistry and Laboratory Medicine : CCLM·Vanessa García MoreiraFrancisco V Alvarez Menéndez
Sep 4, 2007·International Journal of Cancer. Journal International Du Cancer·Jörg EllingerAlexander von Ruecker
Oct 6, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Nobuyasu SuzukiYoshimi Homma
Apr 23, 2008·American Journal of Clinical Pathology·Annalisa AltimariMichelangelo Fiorentino
Jun 4, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Maniesh van der Vaart, Piet J Pretorius
Aug 2, 2008·Nature Medicine·Frank DiehlLuis A Diaz
Aug 13, 2008·DNA and Cell Biology·Raquel CatarinoRui Medeiros
Oct 8, 2008·Annals of the New York Academy of Sciences·Stefan HoldenriederPetra Stieber
Oct 8, 2008·Annals of the New York Academy of Sciences·Anna V CherepanovaPavel P Laktionov
Oct 8, 2008·Annals of the New York Academy of Sciences·Elena V KolesnikovaElena Y Rykova

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