Concentrations of thyroxine, 3,5,3'-triiodothyronine, 3,3',5'-triiodothyronine, 3,3'-diiodothyronine, and 3',5'-diiodothyronine in human red blood cells

Scandinavian Journal of Clinical and Laboratory Investigation
C BregengaardT FRIIS


A simple and rapid method for the estimation of cellular concentration of thyroxine (T4), 3,5,3'-triiodothyronine (T3), 3,3',5'-triiodothyronine (rT3), 3,3'-diiodothyronine (3,3'-T2), and 3',5'-diiodothyronine (3',5'-T2) as well as their distribution between cytosol and membranes in human red blood cells (RBC) is presented. Concentrations of iodothyronines in RBC (RBC-T) were calculated by multiplying the total serum concentrations by the ratio of radioactivity in equal volumes of packed RBCs and serum, pre-incubated with 125I-labelled iodothyronines of high specific activity. Plasma and RBC were separated by centrifugation in capillary glass tubes. The separation of membranes and cystosol was performed by hypotone lysis and centrifugation. The median RBC-T of T4, T3, rT3, 3,3'-T2, and 3',5'-T2 from 17 euthyroid subjects were 360 pmol/l, 156 pmol/l, 2.77 pmol/l, 6.81 pmol/l, and 2.17 pmol/l, respectively. The cytosol/cytosol + membrane ration were 66%, 40%, 84%, 77%, and 97%, respectively. The differences in RBC-T were not similar to the differences in free serum concentrations. The ratio of RBC-T to free serum concentration differed considerably between T4 (16.6), T3 (24.4), and 3,3'-T2 (15.5) as compared to rT3 (5.8) and 3',5...Continue Reading


Jun 27, 1979·Biochemical and Biophysical Research Communications·J MichelotG Meyniel
Jun 1, 1976·Endocrinologia Japonica·Y OchiH Takahashi
Dec 17, 1985·Biochemical and Biophysical Research Communications·J A Botta, R N Farías
Dec 1, 1973·Scandinavian Journal of Clinical and Laboratory Investigation·J Weeke, H Orskov
Jun 1, 1984·The Journal of Clinical Endocrinology and Metabolism·C M Mendel, R R Cavalieri
Jan 1, 1980·Endocrine Research Communications·K Yoshida, P J Davis
Sep 1, 1981·The Journal of Clinical Endocrinology and Metabolism·J FaberT FRIIS
Apr 1, 1980·The Journal of Clinical Endocrinology and Metabolism·K Yoshida, P J Davis

Related Concepts

3,3'-diiodothyronine, (L)-isomer,125I-labeled
Serum Proteins
Thyroid Hormone Receptor

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