Concentrations, viability, and distribution of Cryptosporidium genotypes in lagoons of swine facilities in the Southern Piedmont and in coastal plain watersheds of Georgia.

Applied and Environmental Microbiology
Michael B JenkinsDwight D Bowman

Abstract

Waste lagoons of swine operations are a source of Cryptosporidium oocysts. Few studies, however, have reported on oocyst concentrations in swine waste lagoons; none have reported on oocyst viability status, nor has there been a systematic assessment of species/genotype distributions across different types of swine facilities. Ten swine waste lagoons associated with farrowing, nursery, finishing, and gestation operations were each sampled once a month for a year. Oocysts were extracted from triplicate 900-ml effluent samples, enumerated by microscopy, and assessed for viability by dye exclusion/vital stain assay. DNA was extracted from processed samples, and 18S ribosomal DNA (rDNA) genes were amplified by PCR and sequenced for species and genotype identification. Oocysts were observed at each sampling time at each lagoon. Annual mean concentrations of total oocysts and viable oocysts ranged between 24 and 51 and between 0.6 and 12 oocysts ml(-1) effluent, respectively. The species and genotype distributions were dominated (95 to 100%) by Cryptosporidium suis and Cryptosporidium pig genotype II, the latter of which was found at eight of the lagoons. The lagoon at the gestation facility was dominated by Cryptosporidium muris (90%...Continue Reading

References

Mar 1, 1997·Veterinary Parasitology·M E OlsonT A McAllister
Dec 5, 1998·Applied and Environmental Microbiology·L J RobertsonH V Smith
Dec 29, 1998·Emerging Infectious Diseases·I M SulaimanA A Lal
Jul 31, 1999·Applied and Environmental Microbiology·L XiaoA A Lal
Dec 13, 2000·International Journal for Parasitology·R FayerS J Upton
Jun 27, 2002·The Journal of Infectious Diseases·Lihua XiaoRobert H Gilman
Mar 26, 2003·Veterinary Parasitology·N J GuselleM E Olson
Apr 24, 2003·Journal of Environmental Quality·Tat Yee Guan, Richard A Holley
Jul 4, 2003·Applied and Environmental Microbiology·U M RyanR C A Thompson
May 25, 2004·Methods in Molecular Biology·Lihua XiaoJianlin Jiang
Jul 10, 2004·Letters in Applied Microbiology·M L HutchisonA Moore
Jul 16, 2004·Journal of Environmental Quality·Lowry A HarperF Michael Byers
Sep 11, 2004·The Journal of Parasitology·U M RyanL Xiao
Dec 14, 2005·Applied and Environmental Microbiology·M B JenkinsW C Ghiorse
Apr 20, 2006·Veterinary Parasitology·Ronald FayerJ P Dubey
May 31, 2006·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·J VítovecB Sak
Jun 6, 2006·Applied and Environmental Microbiology·Lihua XiaoJuluri R Rao
Nov 25, 2006·Veterinary Parasitology·Inger Sofie HamnesLucy Jane Robertson
Jan 9, 2008·Bioresource Technology·Roberto Reinoso, Eloy Becares
Jan 22, 2008·FEMS Immunology and Medical Microbiology·Lihua Xiao, Yaoyu Feng
May 20, 2008·Experimental Parasitology·Johanna JohnsonUna M Ryan
Dec 23, 2008·Veterinary Parasitology·Susana Pedraza-DíazMercedes Gómez-Bautista
Apr 11, 2009·Experimental Parasitology·Lihua Xiao

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Citations

Oct 29, 2013·EcoHealth·Juan J Carrique-Mas, J E Bryant
Aug 22, 2012·Parasitology·Una Ryan, Michelle Power
Jun 17, 2011·Foodborne Pathogens and Disease·Abdolvahab FarzanBrent Dixon
Sep 29, 2012·The Journal of Parasitology·Michael B JenkinsDwight D Bowman
Feb 3, 2015·Polish Journal of Veterinary Sciences·A RzeżutkaZ Pejsak

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