Conceptual and methodological issues relevant to cytokine and inflammatory marker measurements in clinical research.

Current Opinion in Clinical Nutrition and Metabolic Care
Xin ZhouGeorge A Kuchel

Abstract

To provide clinical investigators with an understanding of factors to consider when wishing to add cytokine and inflammatory marker measurements to their studies. Inflammation involves complex and coordinated responses of the immune system to tissue damage. In the absence of tools to routinely assess inflammation within living tissues, measurements of humoral factors such as cytokines and other inflammatory mediators or markers can provide predictive clinical information and insights into disease mechanisms. Historically, enzyme-linked immunosorbent assays (ELISAs) became the gold standard, yet this approach of measuring a single protein in each sample limits the amount of information which can be obtained from limited amounts of human sample. In recent years, commercially available multiplex technologies which detect large numbers of proteins in a limited volume have provided investigators with opportunities to begin addressing the complexity of inflammatory responses. Nevertheless, great attention needs to be paid to many aspects of study design, sample collection, sample measurement and data analysis. These considerations are especially significant when using technologies for which experience remains limited. Whereas measure...Continue Reading

References

Aug 30, 1992·Journal of Immunological Methods·P W ThavasuF R Balkwill
Jan 1, 1974·American Journal of Clinical Pathology·G Lum, S R Gambino
Mar 1, 1997·International Journal of Sports Medicine·H Gabriel, W Kindermann
Jun 20, 1998·The Journal of Physiology·K OstrowskiB K Pedersen
Aug 26, 1998·The Journal of Clinical Endocrinology and Metabolism·P DandonaT Wadden
Oct 13, 1998·Toxicology·J A BienvenuN Fabien
Jan 5, 1999·Clinical and Diagnostic Laboratory Immunology·N AzizJ L Fahey
Feb 2, 1999·The Journal of Physiology·K OstrowskiB K Pedersen
Mar 26, 1999·Sports Medicine·D C Nieman, B K Pedersen
May 16, 2000·Critical Reviews in Clinical Laboratory Sciences·R E Banks
Oct 22, 2002·American Journal of Physiology. Endocrinology and Metabolism·Adam SteensbergBente Klarlund Pedersen
Feb 20, 2003·The Journal of Allergy and Clinical Immunology·Larry C Borish, John W Steinke
May 26, 2004·Brain, Behavior, and Immunity·Monika HaackJanet M Mullington
Sep 8, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Martijn A NolteReina E Mebius
May 21, 2005·Neuroimmunomodulation·A N VgontzasG P Chrousos
Feb 17, 2006·Methods : a Companion to Methods in Enzymology·Mohamed F Elshal, J Philip McCoy
Jun 27, 2006·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Marcello MaggioLuigi Ferrucci
Oct 26, 2006·Obesity·Patricia BlackburnCharles Couillard
Apr 24, 2007·Cytokine·Gitika PanickerElizabeth R Unger
Apr 3, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Bridget E McLaughlinDavid M Asmuth
Apr 3, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Bridget E McLaughlinDavid M Asmuth
Jun 21, 2008·Nature Reviews. Immunology·Dario A A VignaliCreg J Workman
Jul 10, 2008·Methods in Molecular Medicine·William P StefuraKent T HayGlass
Aug 20, 2008·Nature Immunology·Marcus Thelen, Jens V Stein
Sep 6, 2008·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Sean X LengGeorge A Kuchel
Jul 4, 2009·Nature Reviews. Immunology·Sarah L Gaffen
Jul 17, 2009·Metabolism: Clinical and Experimental·Caroline PayetteCharles Couillard
Sep 30, 2009·BMC Immunology·Wilco de JagerVicki Seyfert-Margolis
Nov 26, 2009·The Journal of Allergy and Clinical Immunology·Scott P ComminsJohn W Steinke
Feb 24, 2010·Journal of Biomolecular Screening·Joanna L RichensLucy Fairclough
Mar 23, 2010·Cell·Ruslan Medzhitov
Mar 23, 2010·Cell·Carl Nathan, Aihao Ding
Mar 24, 2010·Journal of Theoretical Biology·Jeremy D ScheffIoannis P Androulakis

❮ Previous
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Citations

Jan 20, 2012·Journal of Proteome Research·Stine L BislevEmøke Bendixen
Aug 24, 2013·Journal of the Royal Society, Interface·Wanyu ChenAmy Q Shen
Oct 13, 2011·Journal of Translational Medicine·Lisa H ButterfieldJohn M Kirkwood
Apr 16, 2014·Mediators of Inflammation·Agata BurskaFrederique Ponchel
Mar 7, 2012·Intensive & Critical Care Nursing : the Official Journal of the British Association of Critical Care Nurses·Christina Amidei
May 12, 2015·Critical Reviews in Toxicology·Julie E GoodmanFerdinand J Venditti
Jun 25, 2013·American Journal of Reproductive Immunology : AJRI·Sien Yee LauLawrence W Chamley
Feb 23, 2012·Arthritis and Rheumatism·Hani S El-GabalawyMarvin J Fritzler
Feb 13, 2013·Clinical and Translational Science·Justin E JuskewitchW Charles Huskins
Nov 12, 2013·Journal of Clinical Periodontology·Poliana M DuarteLuciana M Shaddox
Sep 18, 2015·Intensive Care Medicine Experimental·Ignacio Martin-LoechesAntonio Artigas
Sep 27, 2014·Critical Reviews in Toxicology·Robyn L PrueittJulie E Goodman
Sep 27, 2014·Critical Reviews in Toxicology·Julie E GoodmanFerdinand J Venditti
Mar 30, 2013·Journal of Clinical Pathology·David J PinatoMichael A Mendall
Sep 10, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·Thor UelandErik T Askevold
Jan 15, 2015·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Madhu Cholenahalli LingarajuSurendra Kumar Tandan
Dec 4, 2014·Analytica Chimica Acta·Julie A Stenken, Andreas J Poschenrieder
Jan 23, 2014·Pathogens and Disease·Nazly ShafagatiKylene Kehn-Hall
Jun 28, 2016·Journal of Affective Disorders·Seline van den AmeeleManuel Morrens
Mar 25, 2016·Indian Journal of Psychological Medicine·Maju Mathew Koola
Oct 26, 2016·Seminars in Oncology Nursing·Debra Lynch KellyLeorey N Saligan
Dec 29, 2015·Scandinavian Journal of Immunology·D M AresvikT G Abrahamsen
Aug 25, 2016·Journal of Applied Oral Science : Revista FOB·Cassio KampitsAlex N Haas
May 11, 2017·International Journal of Inflammation·Rebecca N Monastero, Srinivas Pentyala

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