Concerns in the application of fluorescent probes DCDHF-DA, DHR 123 and DHE to measure reactive oxygen species in vitro

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Mazyar Yazdani

Abstract

Reactive oxygen species (ROS) are formed in biological systems by partial reduction of molecular oxygen. The essential role of ROS in maintaining physiological health may be corrupted into oxidative stress by their overproduction or the exhaustion of antioxidant mechanisms. Many studies covering a broad range of methodologies have investigated ROS production and their toxic mechanisms of action. Of these methodologies, fluorometry has been among the preferred techniques. Three frequently used fluorescent probes for in vitro studies are 2',7'-dichlorodihydrofluorescein diacetate (DCDHF-DA), Dihydrorhodamine 123 (DHR 123) and Dihydroethidium (DHE). Apart from the unavoidable limitations of auto-oxidation, photo-oxidation and photo-conversion, there are also concerns relating to protocol modification for the improved monitoring of ROS. This paper aims to highlight such contributing factors, including cell culture conditions and the characteristics of individual fluorescent probes in the utilization of these selected probes in in vitro systems.

References

Jan 1, 1971·Experimental Cell Research·J A Dickson
Feb 1, 1994·Free Radical Biology & Medicine·N W KooyJ S Beckman
Aug 31, 1993·Biochemical and Biophysical Research Communications·S MaedaT Saito
Feb 7, 2001·Toxicology·J Pourahmad, P J O'Brien
Jul 11, 2002·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Adrienne N Boone, Mathilakath M Vijayan
Jul 20, 2002·Aquatic Toxicology·Michela FerrarisEnzo Chiesara
Jan 7, 2003·The Journal of Biological Chemistry·Vytautas P BindokasLouis H Philipson
Apr 1, 1965·Analytical Biochemistry·A S KESTON, R BRANDT
Nov 7, 2003·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·K-E TollefsenJ Stenersen
Nov 25, 2003·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Hong LongZhiguo Wang
Sep 4, 2004·Comptes rendus biologies·Olivier Sorg
Nov 22, 2005·Journal of Biochemical and Biophysical Methods·Ana GomesJosé L F C Lima
Jan 3, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·James HynesDmitri B Papkovsky
Jan 10, 2006·Bulletin of Environmental Contamination and Toxicology·M NawazG Krumschnabel
Dec 8, 2007·Aquatic Toxicology·Stephanie K BoppKatja Knauer
Aug 30, 2008·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Daniel RicoJuan-Carlos Gutiérrez
Aug 5, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Sougat Misra, Som Niyogi
Feb 2, 2010·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·E FarmenK E Tollefsen
Nov 30, 2014·Bulletin of Environmental Contamination and Toxicology·Mazyar YazdaniKetil Hylland

❮ Previous
Next ❯

Citations

Oct 21, 2016·Plant Physiology and Biochemistry : PPB·Germán PérezPilar Irisarri
Oct 17, 2017·Toxicological Sciences : an Official Journal of the Society of Toxicology·Assis EckerNilda Vargas Barbosa
Jun 2, 2018·Chemical Communications : Chem Comm·Dan LiXin Li
Oct 3, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cynthia S A CairesAnderson R L Caires
Jun 1, 2017·Chemistry : a European Journal·Diana AndinaPaola Luciani
Jan 11, 2020·Toxicology Reports·Berenice Fernández-RojasGloria Gutiérrez-Venegas
Jul 24, 2021·Seizure : the Journal of the British Epilepsy Association·Mazyar Yazdani, Katja Benedikte Prestø Elgstøen
Aug 11, 2017·Journal of Agricultural and Food Chemistry·Shiau-Han JiangTung-Hu Tsai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.