Concerted actions of a thermo-labile regulator and a unique intergenic RNA thermosensor control Yersinia virulence.

PLoS Pathogens
Katja BöhmePetra Dersch

Abstract

Expression of all Yersinia pathogenicity factors encoded on the virulence plasmid, including the yop effector and the ysc type III secretion genes, is controlled by the transcriptional activator LcrF in response to temperature. Here, we show that a protein- and RNA-dependent hierarchy of thermosensors induce LcrF synthesis at body temperature. Thermally regulated transcription of lcrF is modest and mediated by the thermo-sensitive modulator YmoA, which represses transcription from a single promoter located far upstream of the yscW-lcrF operon at moderate temperatures. The transcriptional response is complemented by a second layer of temperature-control induced by a unique cis-acting RNA element located within the intergenic region of the yscW-lcrF transcript. Structure probing demonstrated that this region forms a secondary structure composed of two stemloops at 25°C. The second hairpin sequesters the lcrF ribosomal binding site by a stretch of four uracils. Opening of this structure was favored at 37°C and permitted ribosome binding at host body temperature. Our study further provides experimental evidence for the biological relevance of an RNA thermometer in an animal model. Following oral infections in mice, we found that tw...Continue Reading

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Citations

Jun 20, 2014·PLoS Pathogens·Halie K MillerVictoria Auerbuch
Dec 21, 2012·Future Microbiology·Rebekka Steinmann, Petra Dersch
May 7, 2013·Future Microbiology·Yanping HanDongsheng Zhou
Oct 15, 2014·Proceedings of the National Academy of Sciences of the United States of America·María Victoria Grosso-BecerraGloria Soberón-Chávez
Sep 18, 2014·Proceedings of the National Academy of Sciences of the United States of America·Gregor G WeberKarl E Klose
May 23, 2015·Trends in Microbiology·María Victoria Grosso-BeceraGloria Soberón-Chávez
Mar 15, 2016·Frontiers in Cellular and Infection Microbiology·Shiyun ChenMatthew S Francis
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Mar 25, 2014·Biochimica Et Biophysica Acta·Stefanie Sandra Krajewski, Franz Narberhaus
Jun 28, 2013·Molecular Microbiology·Annika CimdinsFranz Narberhaus
Sep 13, 2014·Molecular Microbiology·Johannes E RothhardtThilo M Fuchs
Feb 20, 2014·Molecular Microbiology·Mandy Starke, Thilo M Fuchs
Dec 17, 2014·Virulence·Oliver LamChristoph M Tang
Oct 7, 2015·Frontiers in Microbiology·Marc Erhardt, Petra Dersch
Sep 25, 2015·Parasitology·Megan A GreischarNicole Mideo
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Jun 15, 2016·Proceedings of the National Academy of Sciences of the United States of America·Francesco RighettiFranz Narberhaus
Jul 22, 2016·RNA Biology·Ann Kathrin HerovenPetra Dersch
Sep 9, 2016·Annual Review of Microbiology·Anna V Sherwood, Tina M Henkin
Jan 18, 2017·Proceedings of the National Academy of Sciences of the United States of America·Aaron M NussPetra Dersch
Feb 13, 2017·Current Opinion in Microbiology·Kayley H Schulmeyer, Timothy L Yahr

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Methods Mentioned

BETA
PCR
nucleotide exchanges
toeprinting

Software Mentioned

SkanIt
AlphaEaseFC
Mfold

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