Concerted loss of cyclooxygenase and peroxidase activities from prostaglandin H synthase upon proteolytic attack

Prostaglandins
R J Kulmacz

Abstract

Prostaglandin H synthase has two distinct enzymatic activities: a cyclooxygenase that forms PGG2 from arachidonate and a peroxidase that can reduce hydroperoxides, such as PGG2, to the corresponding alcohols. The relative sensitivities of the two synthase activities to proteolytic attack have been examined, using trypsin, chymotrypsin, and proteinase K, all known to attack the native apoprotein in the arg 253 region. The relation between the specific activity of the synthase and the loss of the two activities and the cleavage of the synthase subunit during trypsin digestion was also examined. The cyclooxygenase and peroxidase activities declined in concert throughout room temperature digestions with each of the three proteases. There was no indication of a selective loss of either activity in any of the digestions. In separate digestions with the same preparation of synthase, 3.3% (w/w) proteinase K resulted in more extensive loss of activity (90% decrease after 90 min) than did 3% (w/w) trypsin (70% decrease after 120 min) or 5% (w/w) chymotrypsin (60% decrease after 135 min). In tryptic digestions of synthase preparations with cyclooxygenase specific activity between 16 and 125 k units/mg protein, the fractional loss of cyclo...Continue Reading

References

May 25, 1977·Biochimica Et Biophysica Acta·F J Van der OuderaaD A Van Dorp
Feb 1, 1989·Archives of Biochemistry and Biophysics·R J Kulmacz, K K Wu
Jul 1, 1987·European Journal of Biochemistry·R KartheinH H Ruf
Sep 1, 1986·Archives of Biochemistry and Biophysics·R J Kulmacz
Jul 31, 1985·Biochemical and Biophysical Research Communications·R J KulmaczW E Lands
Jan 29, 1982·Biochemical and Biophysical Research Communications·R J Kulmacz, W E Lands
Apr 1, 1982·Archives of Biochemistry and Biophysics·B G TitusW E Lands

❮ Previous
Next ❯

Citations

Jun 20, 2003·Progress in Lipid Research·Richard J KulmaczAh-Lim Tsai
Apr 22, 2004·The Journal of Biological Chemistry·Wen LiuRichard J Kulmacz
Aug 1, 1997·Archives of Biochemistry and Biophysics·Q GuoR J Kulmacz
Nov 26, 2008·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Alexey GoltsovGalina Lebedeva

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
I D MacDonald, H B Dunford
Investigative Ophthalmology & Visual Science
Sapna GangaputraDiabetic Retinopathy Clinical Research Network
Archives of Biochemistry and Biophysics
R J Kulmacz, K K Wu
Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
L A Marquez, H B Dunford
© 2022 Meta ULC. All rights reserved