Concomitant alterations of sodium flux and membrane phospholipid metabolism in red blood cells: studies in hereditary spherocytosis

The Journal of Clinical Investigation
H Jacob, M L Karnovsky

Abstract

The role of membrane phosphatides in transport processes has been investigated in red cells from splenectomized patients with hereditary spherocytosis (HS). Incorporation of inorganic (32)phosphate into the membrane phosphatides of HS red cells was approximately twice normal, coinciding with the nearly twofold increment in flux of sodium ions in the cells.A consistent, inordinate increase in specific activity of a chromatographic fraction containing phosphatidylserine provided the bulk of the over-all increase in labeling of HS red cell phosphatides. The specific activity of phosphatidic acid was increased but not consistently. Radioactivity of the "acidic phosphatides" (phosphatidylserine and phosphatidic acid fractions) decreased, in general, when the sodium flux was low, i.e., when the cells were suspended in media of low sodium content. When the cation flux was elevated (hypotonic media), there was a marked (ca. 35%) increase in the labeling of phosphatidylserine fractions. Normal red cells whose permeability to cations was increased by exposure to 0.5 N butanol also exhibited increased labeling of acidic phosphatides. Considerations of the stoichiometry of cation transport and phosphatide labeling make it unlikely that pho...Continue Reading

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Citations

Mar 14, 1968·The New England Journal of Medicine·H S ZarkowskyD G Nathan
Mar 16, 1972·The New England Journal of Medicine·S B Shohet
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