Abstract
The myometrial beta-adrenergic receptor (beta-AR)-adenylyl cyclase pathway is markedly desensitized at the end of pregnancy in the rat. We have investigated whether changes in the amount and/or the activity of G protein-coupled receptor kinase (GRK) occurred at the same period of pregnancy. Using Northern and Western blotting, we have identified GRK2, GRK5, GRK6, and a small amount of GRK3 in late pregnant rat myometrium. GRK activity, as measured by in vitro phosphorylation of rhodopsin, was detected in both cytosolic and plasma membrane fractions. Interestingly, in the 6-10 h preceding parturition, there was a substantial increase (+190%) of myometrial membrane-associated GRK activity. This was associated with an increase in membrane GRK2 immunoreactivity. Such alterations occurred concomitantly with uncoupling of beta-AR, as assessed by quantification of high-affinity binding receptors. These data suggest that GRK activity increase may be one of the mechanisms underlying alterations in the coupling between beta-AR and adenylyl cyclase and may thus contribute to the initiation of myometrial contractions at term.
References
May 7, 1976·Analytical Biochemistry·M M Bradford
Apr 1, 1991·Journal of Molecular Endocrinology·J Cohen-TannoudjiJ P Maltier
Jan 1, 1990·Life Sciences·M A CruzM I Rudolph
Jul 1, 1989·The Journal of Clinical Endocrinology and Metabolism·M H LitimeF Ferre
Oct 13, 1989·Science·J L BenovicR J Lefkowitz
Jan 1, 1986·Journal of Reproduction and Fertility·C Legrand, J P Maltier
Mar 8, 1988·Biochemistry·N Bennett, A Sitaramayya
Jul 1, 1995·Biology of Reproduction·J Cohen-TannoudjiJ P Maltier
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·P Kunapuli, J L Benovic
May 5, 1995·The Journal of Biological Chemistry·S MhaoutyC Legrand
Jun 2, 1995·Science·W J KochR J Lefkowitz
Aug 1, 1994·Journal of Molecular Endocrinology·J Elwardy-MérézakC Legrand
Apr 1, 1993·American Journal of Obstetrics and Gynecology·K HigbyC J Pauerstein
Mar 1, 1994·The Journal of Clinical Investigation·I García-Higuera, F Mayor
Feb 1, 1994·Circulation Research·M UngererM J Lohse
Jan 1, 1993·The American Journal of Physiology·S Wray
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·M OppermannR J Lefkowitz
Sep 1, 1996·Circulation Research·M UngererG Richardt
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·H A RockmanW J Koch
May 1, 1997·American Journal of Obstetrics and Gynecology·A L Ruzycky, J A DeLoia
Oct 8, 1997·The American Journal of Physiology·D Firsov, J M Elalouf
May 26, 1998·Seminars in Cell & Developmental Biology·S S Ferguson, M G Caron
Jun 2, 1998·Endocrinology·B VirlonJ M Elalouf
Jul 23, 1998·Biology of Reproduction·J L LécrivainJ P Maltier
Oct 6, 1998·Annual Review of Biochemistry·J A PitcherR J Lefkowitz
Feb 3, 1999·Molecular Pharmacology·S Mhaouty-KodjaS Cotecchia
Sep 1, 1999·The Journal of Endocrinology·C B BrenninkmeijerS Phaneuf
Citations
Feb 28, 2003·Biology of Reproduction·G CharpignyG Germain
Feb 11, 2004·British Journal of Pharmacology·C RougetM Bardou
Aug 28, 2002·Circulation·Ruth LandauRichard M Smiley
Jun 18, 2003·Endocrinology·Violaine SimonJoëlle Cohen-Tannoudji
May 9, 2009·Molecular Endocrinology·Jonathon M WilletsR A John Challiss
Oct 23, 2009·Endocrinology·Elisa K TysonMark Read
Dec 18, 2004·Endocrinology·Xiaoping LuoNasser Chegini
Nov 5, 2015·Physiological Genomics·Sreenath ChandranTerry J Smith
Feb 19, 2004·The Journal of Biological Chemistry·Morgan DupuisSakina Mhaouty-Kodja