Concomitant increase of G protein-coupled receptor kinase activity and uncoupling of beta-adrenergic receptors in rat myometrium at parturition

Endocrinology
Violaine SimonJoëlle Cohen-Tannoudji

Abstract

The myometrial beta-adrenergic receptor (beta-AR)-adenylyl cyclase pathway is markedly desensitized at the end of pregnancy in the rat. We have investigated whether changes in the amount and/or the activity of G protein-coupled receptor kinase (GRK) occurred at the same period of pregnancy. Using Northern and Western blotting, we have identified GRK2, GRK5, GRK6, and a small amount of GRK3 in late pregnant rat myometrium. GRK activity, as measured by in vitro phosphorylation of rhodopsin, was detected in both cytosolic and plasma membrane fractions. Interestingly, in the 6-10 h preceding parturition, there was a substantial increase (+190%) of myometrial membrane-associated GRK activity. This was associated with an increase in membrane GRK2 immunoreactivity. Such alterations occurred concomitantly with uncoupling of beta-AR, as assessed by quantification of high-affinity binding receptors. These data suggest that GRK activity increase may be one of the mechanisms underlying alterations in the coupling between beta-AR and adenylyl cyclase and may thus contribute to the initiation of myometrial contractions at term.

References

Jul 1, 1989·The Journal of Clinical Endocrinology and Metabolism·M H LitimeF Ferre
Jan 1, 1986·Journal of Reproduction and Fertility·C Legrand, J P Maltier
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·P Kunapuli, J L Benovic
Apr 1, 1993·American Journal of Obstetrics and Gynecology·K HigbyC J Pauerstein
Mar 1, 1994·The Journal of Clinical Investigation·I García-Higuera, F Mayor
Jan 1, 1993·The American Journal of Physiology·S Wray
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·M OppermannR J Lefkowitz
Sep 1, 1996·Circulation Research·M UngererG Richardt
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·H A RockmanW J Koch
May 1, 1997·American Journal of Obstetrics and Gynecology·A L Ruzycky, J A DeLoia
Oct 8, 1997·The American Journal of Physiology·D Firsov, J M Elalouf
May 26, 1998·Seminars in Cell & Developmental Biology·S S Ferguson, M G Caron
Oct 6, 1998·Annual Review of Biochemistry·J A PitcherR J Lefkowitz
Sep 1, 1999·The Journal of Endocrinology·C B BrenninkmeijerS Phaneuf

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Citations

Feb 19, 2004·The Journal of Biological Chemistry·Morgan DupuisSakina Mhaouty-Kodja

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