Concomitant synthesis of bacteriocin and bacteriocin inactivator from Serratia marcescens.

Journal of Bacteriology
J D Foulds, D Shemin

Abstract

We have found that Serratia marcescens strain P & S is bacteriocinogenic. However, the phenotypic expression of bacteriocin activity depends upon the temperature at which the cells are grown. When the organism is grown at 30 to 37 C, no bacteriocin activity can be demonstrated, whereas when it is grown at 39 C bacteriocin activity is readily observed. It appears that the P & S strain concomitantly synthesizes a bacteriocin and a substance which not only can inactivate the bacteriocin but also has a high activation energy for inactivation. This inactivator readily loses its activity when heated at 39 C for 1 hr. Two mutants were isolated from the P & S strain which can produce active bacteriocin when grown at temperatures from 30 to 39 C. It is significant that these mutants have considerably less bacteriocin inactivator. The data suggest that the inactivator is an extracellular protease. The ability of one of these mutants, JF58-12, to produce active bacteriocin at temperatures between 30 and 39 C is a stable property, whereas in the other mutant, JF48W, this property is unstable. JF48W was selected from the P & S strain in two steps: first a streptomycin-resistant variant (strain A-10) was isolated and from this mutant a strai...Continue Reading

References

Jan 1, 1967·Annual Review of Microbiology·M Nomura
Sep 1, 1969·Journal of Bacteriology·J D Foulds, D Shemin
Jan 1, 1957·Annual Review of Microbiology·P FREDERICQ
Apr 23, 1963·Biochemical and Biophysical Research Communications·B L REYNOLDS, P R REEVES
May 4, 1962·Biochemical and Biophysical Research Communications·M NOMURA, M NAKAMURA

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Citations

Sep 1, 1971·Journal of Bacteriology·J Foulds
Sep 1, 1976·Bacteriological Reviews·J R TaggL W Wannamaker
Apr 1, 1977·Infection and Immunity·H Farkas-Himsley, A Pagel
Jun 1, 1973·Journal of Bacteriology·S K Guterman
Sep 1, 1969·Journal of Bacteriology·J D Foulds, D Shemin
Sep 1, 1973·Antimicrobial Agents and Chemotherapy·J R TaggA R McGiven

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