Concomitant treatment with pertussis toxin plus temozolomide increases the survival of rats bearing intracerebral RG2 glioma

Journal of Cancer Research and Clinical Oncology
Roxana Magaña-MaldonadoBenjamín Pineda

Abstract

Glioblastoma multiforme is the most frequent primary brain tumor, it has poor prognosis, and it remains refractory to current treatment. The success of temozolomide (TMZ) appears to be limited by the occurrence of chemoresistance. Recently, we report the use of pertussis toxin as adjuvant immunotherapy in a C6 glioma model; showing a decrease in tumoral size, it induced selective cell death in Treg cells, and it elicited less infiltration of tumoral macrophages. Here, we evaluated the cytotoxic effect of pertussis toxin in combination with TMZ for glioma treatment, both in vitro and in vivo RG2 glioma model. We determined cell viability, cell cycle, apoptosis, and autophagy on treated RG2 cells through flow cytometry, immunofluorescence, and Western blot assays. Twenty-eight rats were divided in four groups (n = 7) for each treatment. After intracranial implantation of RG2 cells, animals were treated with TMZ (10 mg/Kg/200 μl of apple juice), PTx (2 μg/200 μl of saline solution), and TMZ + PTx. Animals without treatment were considered as control. We found an induction of apoptosis in around 20 % of RG2 cells, in both single treatments and in their combination. Also, we determined the presence of autophagy vesicles, without any...Continue Reading

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Citations

Feb 3, 2016·Journal of Experimental & Clinical Cancer Research : CR·Yuanliang YanZhicheng Gong
May 21, 2016·Frontiers in Immunology·Aleli Salazar-RamiroBenjamín Pineda
Jun 14, 2016·BioMed Research International·Roxana Magaña-MaldonadoBenjamín Pineda
Aug 24, 2017·Oxidative Medicine and Cellular Longevity·Daniela Ramírez-OrtegaVerónica Pérez de la Cruz
Sep 22, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Fan FengXiujie Wu

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