Concordance between Immunohistochemistry and Microarray Gene Expression Profiling for Estrogen Receptor, Progesterone Receptor, and HER2 Receptor Statuses in Breast Cancer Patients in Lebanon

International Journal of Breast Cancer
Ghina B FakhriArafat H Tfayli

Abstract

Accurate evaluation of estrogen and progesterone receptors and HER2 is critical when diagnosing invasive breast cancer for optimal treatment. The current evaluation method is via immunohistochemistry (IHC). In this paper, we compared results of ER, PR, and HER2 from microarray gene expression to IHC in 81 fresh breast cancer specimens. Gene expression profiling was performed using the GeneChip Human Genome U133 Plus 2.0 arrays (Affymetrix Inc). Immunohistochemical staining for estrogen receptor, progesterone receptor, and HER2 status was performed using standard methods at a CAP-accredited pathology laboratory. Concordance rates, agreement measures, and kappa scores were calculated for both methods. For ER, Kappa score was 0.918 (95% CI, 0.77.3-1.000) and concordance rate was 97.5% (95% CI, 91.4%-99.7%). For PR, Kappa score was 0.652 (95% CI, 0.405-0.849) and concordance rate was 86.4% (95% CI, 77%-93%). For HER2, Kappa score was 0.709 (95% CI, 0.428-0.916) and concordance rate was 97.5% (95% CI, 91.4%-99.7%). Our results are in line with the available evidence with the concordance rate being the lowest for the progesterone receptor. In general, microarray gene expression and IHC proved to have high concordance rates. Several f...Continue Reading

References

Sep 13, 2001·Proceedings of the National Academy of Sciences of the United States of America·T SørlieA L Børresen-Dale
Apr 1, 2003·Acta Oncologica·Gunilla ChebilUNKNOWN North Sweden Breast Cancer Group
Jun 28, 2006·Clinical Breast Cancer·Josina C ReddyPamela M Klein
Jul 1, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Edith A PerezRobert B Jenkins
May 9, 2007·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Tetsunari OyamaJun Horiguchi
Oct 24, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Lyndsay HarrisUNKNOWN American Society of Clinical Oncology
May 20, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·D Craig Allred
Jun 12, 2008·Journal of the National Cancer Institute·Karyn Hede
Apr 7, 2009·Indian Journal of Medical Sciences·Lakmini Mudduwa, Thusharie Liyanage
Nov 6, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Paul RoepmanMarc J van de Vijver
Dec 22, 2011·Journal of the National Comprehensive Cancer Network : JNCCN·Phillip G FebboMarian L Birkeland
Mar 29, 2014·Annals of Oncology : Official Journal of the European Society for Medical Oncology·G VialeUNKNOWN TRANSBIG Consortium & the MINDACT Investigators
Jul 6, 2016·Virchows Archiv : an International Journal of Pathology·Jelle WesselingPino Cusumano
Jan 6, 2017·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Apr 1, 2014·Annals of Oncology : Official Journal of the European Society for Medical Oncology·G VialeUNKNOWN TRANSBIG Consortium & the MINDACT Investigators

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Citations

Feb 13, 2020·NPJ Breast Cancer·Dennis CaruanaEmily S Reisenbichler
Feb 5, 2021·NPJ Breast Cancer·Dennis CaruanaEmily S Reisenbichler

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Methods Mentioned

BETA
Electrophoresis
PCR
Fluorescence

Software Mentioned

Affymetrix GeneChip Command Console ( AGCC )
TargetPrint
HercepTest
OncotypeDx

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