Concurrent analysis of neuropeptides and biogenic amines in brain tissue of rats treated with electroconvulsive stimuli

Brain Research
C StenforsE Theodorsson

Abstract

We developed a method for measuring neuropeptides and monoamines in the same rat brain tissue and applied this method to study the effects of electroconvulsive stimuli (ECS) on these compounds. Rats were treated with repeated ECS or sham ECS. After sacrifice by focused microwave irradiation, brains were dissected and immediately frozen. The tissues were extracted in acetic acid. After lyophilization the samples were reconstituted in phosphate buffer and divided in three fractions: (1) was further purified on a cation-exchange column before catecholamines were measured on a high-performance liquid chromatography (HPLC) system, (2) for measuring serotonin on the HPLC system, (3) for measuring peptide concentrations by specific radioimmunoassays. Confirming our previous findings, ECS significantly increased neuropeptide Y-like immunoreactivity (-LI) in hippocampus and frontal cortex and neurokinin A-LI in the hippocampus, while no changes in substance P- and neurotensin-LI were detected. New findings were a decrease in noradrenaline concentrations in the frontal and occipital cortex and hippocampus, an increase in dopamine concentrations in the frontal and occipital cortex and no serotonin change. In summary, we have developed met...Continue Reading

References

Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·V Chan-PalayS L Palay
Sep 1, 1992·Progress in Neuro-psychopharmacology & Biological Psychiatry·C StenforsA A Mathé
Jul 1, 1992·Journal of Neurochemistry·P S WiddowsonA E Halaris
Jan 1, 1990·Annals of the New York Academy of Sciences·C WahlestedtD J Reis
Jan 1, 1990·Life Sciences·A A MathèE Theodorsson
Aug 30, 1985·Biochemical and Biophysical Research Communications·E Theodorsson-NorheimP G Lindgren
Jan 14, 1987·Neuroscience Letters·M VallejoS L Lightman
Jun 1, 1985·Scandinavian Journal of Clinical and Laboratory Investigation·E Theodorsson-NorheimJ M Lundberg
Jul 1, 1983·Regulatory Peptides·E Theodorsson-Norheim, S Rosell
Jan 1, 1994·Progress in Neuro-psychopharmacology & Biological Psychiatry·C StenforsE Theodorsson
Feb 1, 1993·Neurochemistry International·R Bellmann, G Sperk

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Citations

Feb 28, 2001·European Journal of Pharmacology·P W BurnetP J Harrison
Jun 24, 2009·International Journal of Occupational Medicine and Environmental Health·Radosław SwierczWojciech Wasowicz

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