Concurrent and orthogonal gold(I) and ruthenium(II) catalysis inside living cells

Nature Communications
Cristian VidalJosé L Mascareñas

Abstract

The viability of building artificial metabolic pathways within a cell will depend on our ability to design biocompatible and orthogonal catalysts capable of achieving non-natural transformations. In this context, transition metal complexes offer unique possibilities to develop catalytic reactions that do not occur in nature. However, translating the potential of metal catalysts to living cells poses numerous challenges associated to their biocompatibility, and their stability and reactivity in crowded aqueous environments. Here we report a gold-mediated C-C bond formation that occurs in complex aqueous habitats, and demonstrate that the reaction can be translated to living mammalian cells. Key to the success of the process is the use of designed, water-activatable gold chloride complexes. Moreover, we demonstrate the viability of achieving the gold-promoted process in parallel with a ruthenium-mediated reaction, inside living cells, and in a bioorthogonal and mutually orthogonal manner.

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Citations

May 16, 2019·Organic & Biomolecular Chemistry·Durgadas CherukaraveeduAnnamaria Lilienkampf
Jun 13, 2019·Chembiochem : a European Journal of Chemical Biology·María Tomás-Gamasa, José Luis Mascareñas
Mar 13, 2020·Angewandte Chemie·Soraya Learte-AymamíJosé L Mascareñas
Oct 18, 2019·Nature Catalysis·María Sancho-AlberoAsier Unciti-Broceta
Dec 14, 2018·Chemical Science·Thomas L BrayAsier Unciti-Broceta
May 18, 2020·Journal of Molecular Modeling·Xinghui ZhangTeng Niu
Jul 7, 2020·Angewandte Chemie·Joan Miguel-ÁvilaJosé L Mascareñas
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May 1, 2021·Frontiers in Bioengineering and Biotechnology·Qinyue GaoDongqing Wang
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May 12, 2021·Nature Nanotechnology·Zhaowei ChenZhen Gu
May 11, 2021·Angewandte Chemie·Alejandro Gutiérrez-GonzálezJosé L Mascareñas
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May 29, 2020·Journal of the American Chemical Society·Rui HuangVincent M Rotello
Mar 21, 2019·Journal of the American Chemical Society·Cristian VidalJosé L Mascareñas
Aug 22, 2021·Angewandte Chemie·Philipp LohnerClaudia Jessen-Trefzer

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Methods Mentioned

BETA
amidation
NMR
Fluorescence
fluorescence microscopy
column chromatography
flow cytometry

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