Concurrent binding of complexin and synaptotagmin to liposome-embedded SNARE complexes.

Biochemistry
Michael C Chicka, Edwin R Chapman

Abstract

Synaptotagmin and complexin regulate SNARE-mediated synaptic vesicle exocytosis. It has been proposed that complexin clamps membrane fusion and that Ca(2+)-synaptotagmin displaces complexin from SNARE complexes to relieve this clamping activity. Using a reconstituted system, we demonstrate that complexin and synaptotagmin simultaneously bind to neuronal SNARE complexes and that both apo-synaptotagmin and complexin inhibit SNARE-mediated membrane fusion. Moreover, the clamping ability of apo-synaptotagmin occluded the clamping activity of complexin until the arrival of a Ca(2+) trigger, at which point synaptotagmin accelerated fusion while high concentrations of complexin inhibited fusion. Thus, the inhibitory patterns of synaptotagmin and complexin are different, suggesting that SNAREs assemble into distinct states along the fusion pathway. These data also suggest that during synaptotagmin-regulated vesicle-vesicle fusion, complexin does not function as a fusion clamp that is relieved by Ca(2+)-synaptotagmin.

References

Apr 7, 1998·Cell·T WeberJ E Rothman
Feb 8, 2002·Neuron·Xiaocheng ChenJosep Rizo
Jun 24, 2006·Science·Claudio G GiraudoJames E Rothman
Jul 18, 2006·Nature Structural & Molecular Biology·Johanna R SchaubJames A McNew
Feb 27, 2007·Journal of Molecular Biology·Han DaiJosep Rizo
Sep 11, 2007·Nature Structural & Molecular Biology·Mingshan XueChristian Rosenmund
Sep 18, 2007·Nature Neuroscience·Sarah Huntwork, J Troy Littleton
Feb 16, 2008·Annual Review of Biochemistry·Edwin R Chapman
May 24, 2008·The Journal of Biological Chemistry·Claudio G GiraudoJames E Rothman
May 29, 2008·Proceedings of the National Academy of Sciences of the United States of America·Mingshan XueKerstin Reim
Jul 4, 2008·Nature Structural & Molecular Biology·Leonid V Chernomordik, Michael M Kozlov
Jul 16, 2008·Nature Structural & Molecular Biology·Michael C ChickaEdwin R Chapman

❮ Previous
Next ❯

Citations

Feb 23, 2010·Nature Structural & Molecular Biology·Ucheor B ChoiKeith R Weninger
Oct 15, 2009·Proceedings of the National Academy of Sciences of the United States of America·Christopher StroupeWilliam Wickner
Jun 4, 2010·The Journal of Biological Chemistry·Jerome Di GiovanniMichael Seagar
Jan 10, 2008·Annual Review of Cell and Developmental Biology·Jakob B Sørensen
Dec 15, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ramon A JorqueraJ Troy Littleton
Apr 2, 2014·The Journal of Cell Biology·Madhurima DharaDieter Bruns
Sep 18, 2014·Nature Communications·David SneadDavid Eliezer
Feb 13, 2013·The Journal of Physiology·Ming-Yi LinRobert H Chow
Jan 26, 2016·Nature Reviews. Neuroscience·Thorsten Trimbuch, Christian Rosenmund
Feb 7, 2012·Progress in Neurobiology·Katherine M BetkeHeidi E Hamm
Mar 5, 2016·Biological & Pharmaceutical Bulletin·Satoshi TadokoroNaoko Utsunomiya-Tate
Aug 8, 2015·Cellular and Molecular Life Sciences : CMLS·Ralf MohrmannDieter Bruns
Jul 13, 2013·Journal of Molecular Biology·Junjie XuJosep Rizo
Jan 29, 2013·Neuron·Rachel T WraggJeremy S Dittman
Jan 11, 2011·Current Biology : CB·Robert J HobsonErik M Jorgensen
Jan 11, 2011·Current Biology : CB·Jesse A MartinJeremy S Dittman
May 5, 2011·Biophysical Journal·Elizabeth A Smith, James C Weisshaar
May 25, 2018·Annual Review of Biophysics·Axel T BrungerQiangjun Zhou
Jul 26, 2018·ELife·Mazen MakkeDieter Bruns
Sep 11, 2019·Nature Communications·Nicholas A CourtneyEdwin R Chapman
May 1, 2018·Trends in Cell Biology·Axel T BrungerYing Lai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Nature Structural & Molecular Biology
Johanna R SchaubJames A McNew
Proceedings of the National Academy of Sciences of the United States of America
Jörg MalsamThomas H Söllner
© 2021 Meta ULC. All rights reserved