PMID: 8609376Feb 1, 1996Paper

Concurrent immunohistochemical staining of tumor-infiltrating lymphocytes and carcinoma-associated T (Thomsen-Friedenreich)/Tn antigens in human breast carcinoma

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
B L WangM W Kaufman

Abstract

The composition of tumor-infiltrating lymphocytes (TIL) often reflects the host's immune response to the tumor. To study the relationship of TIL and carcinoma-associated T/Tn antigens in breast carcinoma, a straightforward concurrent immunoenzyme staining procedure was developed. Fresh tissue was directly fixed in a zinc-based fixative to preserve lymphocyte markers and then routinely embedded in paraffin. The TIL subtypes in the sections were identified in the first immunostaining cycle by reaction with a monoclonal antibody (MAb) to lymphocyte markers CD3, CD4, CD8, CD19, or CD56, followed by a modified avidin-biotin procedure and diaminobenzidine tetrahydrochloride-H2O2 for color development. This was followed by paraformaldehyde fixation to block antibody crossreactivity. The T and Tn antigens on carcinoma cells were then demonstrated in a second staining cycle by reaction with an MAb against T or Tn antigen, followed by an indirect immunoalkaline phosphatase procedure and corresponding substrate systems for color development. The distinguishable brown color for TIL and blue or red color for T or Tn antigen enabled us to identify the TIL subsets and to describe their relations with T/Tn antigen expression in situ. This appr...Continue Reading

Citations

Jul 12, 2016·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Mohammad W Khan, Viktor Umansky
Oct 6, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·B L WangS C Carlstedt
Jun 29, 2004·International Journal of Cancer. Journal International Du Cancer·John SotiriadisYoon Berm Kim
Aug 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Julia A CoronellaEvan M Hersh
Jun 17, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Kenneth WesterFredrik Pontén

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