Condensed complexes and the calorimetry of cholesterol-phospholipid bilayers

Biophysical Journal
T G Anderson, Harden McConnell

Abstract

A recent thermodynamic model describes a reversible reaction between cholesterol (C) and phospholipid (P) to form a condensed complex C(nq)P(np). Here q and p are relatively prime integers used to define the stoichiometric composition, and n is a measure of cooperativity. The present study applies this model to the scanning calorimetry of binary mixtures of cholesterol and saturated phosphatidylcholines, especially work by McElhaney and collaborators. These mixtures generally show two heat capacity peaks, a sharp peak and a broad peak. The sharp heat absorption is largely due to the chain melting transition of pure phospholipid. In the present work the broad heat absorption is attributed to the thermal dissociation of complexes. The best fits of the model to the data require the complex formation to be highly cooperative, with cooperativity n = 12. Detailed comparisons are made between model calculations and calorimetric data. A number of unusual features of the data arise naturally in the model. The principal discrepancy between the calculations and experimental results is a spurious calculated heat absorption peak. This discrepancy is related to the reported relative magnitudes of the integrated broad and sharp heat absorptio...Continue Reading

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Citations

Jan 11, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Jianbing ZhangSteven L Regen
May 19, 2004·Nucleic Acids Research·Maria ManuntaAndrew J T George
Mar 25, 2011·Journal of Pharmacy & Bioallied Sciences·Antonio RaudinoMartina Pannuzzo
May 19, 2007·The Journal of Chemical Physics·Arun Radhakrishnan, Harden McConnell
Jan 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Harden McConnell, Arun Radhakrishnan
Aug 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Arun Radhakrishnan, Harden McConnell
Oct 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Arun Radhakrishnan, Harden M McConnell
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