Condensin HEAT subunits required for DNA repair, kinetochore/centromere function and ploidy maintenance in fission yeast

PloS One
Xingya XuMitsuhiro Yanagida

Abstract

Condensin, a central player in eukaryotic chromosomal dynamics, contains five evolutionarily-conserved subunits. Two SMC (structural maintenance of chromosomes) subunits contain ATPase, hinge, and coiled-coil domains. One non-SMC subunit is similar to bacterial kleisin, and two other non-SMC subunits contain HEAT (similar to armadillo) repeats. Here we report isolation and characterization of 21 fission yeast (Schizosaccharomyces pombe) mutants for three non-SMC subunits, created using error-prone mutagenesis that resulted in single-amino acid substitutions. Beside condensation, segregation, and DNA repair defects, similar to those observed in previously isolated SMC and cnd2 mutants, novel phenotypes were observed for mutants of HEAT-repeats containing Cnd1 and Cnd3 subunits. cnd3-L269P is hypersensitive to the microtubule poison, thiabendazole, revealing defects in kinetochore/centromere and spindle assembly checkpoints. Three cnd1 and three cnd3 mutants increased cell size and doubled DNA content, thereby eliminating the haploid state. Five of these mutations reside in helix B of HEAT repeats. Two non-SMC condensin subunits, Cnd1 and Cnd3, are thus implicated in ploidy maintenance.

References

Feb 1, 1991·Research in Microbiology·S HiragaA Jaffé
Dec 1, 1988·Nature·O Niwa, M Yanagida
Oct 1, 1995·Nature Genetics·M A Andrade, P Bork
Oct 1, 1994·Molecular Biology of the Cell·K TakahashiM Yanagida
Jun 1, 1993·Genes & Development·N KinoshitaM Yanagida
Jan 1, 1996·Annual Review of Cell and Developmental Biology·D Koshland, A Strunnikov
Feb 27, 1997·Nature·C PetosaR C Liddington
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·X HeS Sazer
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·H S MalikD S Goldfarb
Jun 6, 1998·Genes & Development·R A BrittonA D Grossman
Aug 24, 1999·Methods : a Companion to Methods in Enzymology·M P Arroyo, T S Wang
Apr 6, 2000·Molecular Biology of the Cell·I I OuspenskiB R Brinkley
Mar 27, 2001·Current Biology : CB·S SteffensenC E Sunkel
Feb 21, 2002·Molecular Biology of the Cell·Needhi BhallaAndrew W Murray
May 10, 2002·Nature·Nobuki AonoMitsuhiro Yanagida
Jan 30, 2003·Cell·Jason Perry, Nancy Kleckner
Aug 30, 2003·Trends in Cell Biology·Juliet C Coates
Oct 1, 2003·The EMBO Journal·Thilo RiedlJean-Marc Egly
May 18, 2004·Molecular Biology of the Cell·Takao OnoTatsuya Hirano

❮ Previous
Next ❯

Citations

Feb 20, 2017·Current Opinion in Cell Biology·Kazuhisa Kinoshita, Tatsuya Hirano
Dec 17, 2017·Scientific Reports·Beatriz Navarro-DomínguezMaría Dolores López-León
May 8, 2018·Proceedings of the National Academy of Sciences of the United States of America·Xingya XuMitsuhiro Yanagida
May 11, 2019·Proceedings of the National Academy of Sciences of the United States of America·Xingya Xu, Mitsuhiro Yanagida

❮ Previous
Next ❯

Methods Mentioned

BETA
dissections
PCR
dissection
fluorescence-activated cell sorting
FACS
fluorescence microscopy
PCRs

Software Mentioned

Radius
BZ
II Analyzer

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.