Condensin II Regulates Interphase Chromatin Organization Through the Mrg-Binding Motif of Cap-H2

G3 : Genes - Genomes - Genetics
Heather A WallaceGiovanni Bosco

Abstract

The spatial organization of the genome within the eukaryotic nucleus is a dynamic process that plays a central role in cellular processes such as gene expression, DNA replication, and chromosome segregation. Condensins are conserved multi-subunit protein complexes that contribute to chromosome organization by regulating chromosome compaction and homolog pairing. Previous work in our laboratory has shown that the Cap-H2 subunit of condensin II physically and genetically interacts with the Drosophila homolog of human MORF4-related gene on chromosome 15 (MRG15). Like Cap-H2, Mrg15 is required for interphase chromosome compaction and homolog pairing. However, the mechanism by which Mrg15 and Cap-H2 cooperate to maintain interphase chromatin organization remains unclear. Here, we show that Cap-H2 localizes to interband regions on polytene chromosomes and co-localizes with Mrg15 at regions of active transcription across the genome. We show that co-localization of Cap-H2 on polytene chromosomes is partially dependent on Mrg15. We have identified a binding motif within Cap-H2 that is essential for its interaction with Mrg15, and have found that mutation of this motif results in loss of localization of Cap-H2 on polytene chromosomes and...Continue Reading

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Methods Mentioned

BETA
ChIP-seq
transfections
PCR
electrophoresis
transgenic
immunoprecipitation
Fluorescence
ChIP

Software Mentioned

ImageJ
Nikon Elements
Excel
Integrated Genome Viewer ( IGV )

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