Conditional expression of vaccinia virus genes in mammalian cell lines expressing the tetracycline repressor

Journal of Virological Methods
Marika Hedengren-Olcott, D E Hruby

Abstract

A new system was developed for producing conditional-lethal vaccinia virus mutants using the tetracycline controlled gene expression system from bacteria. The tetracycline resistance operon (tetO) sequence was placed between the promoter and coding sequence of the target gene of the virus. To regulate the expression of the target gene, the tetO-containing virus was used to infect a tetracycline repressor (TetR) expressing cell line. This method allowed isolation of a tetO-containing virus in the absence of the TetR thereby eliminating the risk of selecting for an inactivated viral tetR gene caused by recombination of the virus genome and improving the stability of the engineered mutants.

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Citations

Aug 28, 2004·Journal of Virology·Marika Hedengren-OlcottDennis E Hruby
Nov 7, 2006·BMC Biotechnology·Alaka MullickBernard Massie
Dec 13, 2005·Virology Journal·Robert E BlouchDennis E Hruby
Mar 1, 2006·Virology Journal·Kady M HoneychurchDennis E Hruby
Apr 20, 2018·Viruses·Robert WitteUrs F Greber

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