PMID: 12756187May 21, 2003Paper

Conditional inactivation of FGF receptor 2 reveals an essential role for FGF signaling in the regulation of osteoblast function and bone growth

Development
Kai YuDavid M Ornitz

Abstract

Human craniosynostosis syndromes, resulting from activating or neomorphic mutations in fibroblast growth factor receptor 2 (FGFR2), underscore an essential role for FGFR2 signaling in skeletal development. Embryos harboring homozygous null mutations in FGFR2 die prior to skeletogenesis. To address the role of FGFR2 in normal bone development, a conditional gene deletion approach was adopted. Homologous introduction of cre recombinase into the Dermo1 (Twist2) gene locus resulted in robust expression of CRE in mesenchymal condensations giving rise to both osteoblast and chondrocyte lineages. Inactivation of a floxed Fgfr2 allele with Dermo1-cre resulted in mice with skeletal dwarfism and decreased bone density. Although differentiation of the osteoblast lineage was not disturbed, the proliferation of osteoprogenitors and the anabolic function of mature osteoblasts were severely affected.

References

Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·T MikiS A Aaronson
Jun 13, 1991·Biochimica Et Biophysica Acta·M MetsärantaE Vuorio
Oct 1, 1970·The New England Journal of Medicine·D L RimoinR Silberberg
Jun 21, 1996·The Journal of Biological Chemistry·D M OrnitzM Goldfarb
Jan 1, 1997·Human Molecular Genetics·A O Wilkie
Jul 3, 1998·The EMBO Journal·P W Ingham
Oct 24, 1998·Mechanisms of Development·A L DelezoideJ Bonaventure
Jan 23, 1999·Nature Genetics·K SekineS Kato
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y WangD Givol
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·K YuD M Ornitz
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·K Yu, D M Ornitz
Mar 29, 2001·Genome Biology·D M Ornitz, N Itoh
Apr 9, 2002·Genes & Development·Zhonghao LiuDavid M Ornitz
Apr 24, 2002·Developmental Cell·Gerard Karsenty, Erwin F Wagner
Jul 30, 2002·The Journal of Biological Chemistry·David M WillisDwight A Towler

❮ Previous
Next ❯

Citations

Mar 25, 2010·Calcified Tissue International·Vijay K YadavPatricia Ducy
Mar 2, 2011·In Vitro Cellular & Developmental Biology. Animal·T KizhnerE Livne
Oct 1, 2013·Nature Medicine·Jean B RegardYingzi Yang
Jan 23, 2010·Nature Reviews. Cancer·Nicholas Turner, Richard Grose
Dec 23, 2011·Nature Reviews. Molecular Cell Biology·Fanxin Long
Jan 6, 2010·The Journal of Biological Chemistry·Siddharth R VoraPhilip C Trackman
Dec 6, 2008·The Journal of Biological Chemistry·Liping XiaoMarja M Hurley
Feb 25, 2011·Molecular Biology of the Cell·Marcus WatkinsRoberto Civitelli
Sep 4, 2013·Cardiovascular Research·Carsten RudatAndreas Kispert
Feb 13, 2009·Development, Growth & Differentiation·Hisashi Umemori
Sep 19, 2007·Molecular and Cellular Biology·Enrique C TorchiaSuzanne J Baker
Dec 20, 2011·American Journal of Respiratory Cell and Molecular Biology·Emma L Rawlins, Anne-Karina Perl
Oct 2, 2012·The Journal of Clinical Investigation·Caterina TiozzoParviz Minoo
May 16, 2012·The Journal of Clinical Investigation·Yingli WangEthylin Wang Jabs
Jul 26, 2008·Development·Michael J GeskeThaddeus S Stappenbeck
Dec 15, 2010·Development·Frédéric G SalaSaverio Bellusci
Nov 24, 2011·Development·Jirouta KitagakiAlan O Perantoni
Mar 10, 2011·Development·Anyonya R GunturMichael C Naski
Feb 26, 2011·Development·Sunder Sims-LucasCarlton M Bates

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Signaling by Tyrosine Kinases

Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. RTKs have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Discover the latest research on cell signaling and RTK here.