Conditional knockout of TMEM16A/anoctamin1 abolishes the calcium-activated chloride current in mouse vomeronasal sensory neurons

The Journal of General Physiology
Asma AmjadAnna Menini

Abstract

Pheromones are substances released from animals that, when detected by the vomeronasal organ of other individuals of the same species, affect their physiology and behavior. Pheromone binding to receptors on microvilli on the dendritic knobs of vomeronasal sensory neurons activates a second messenger cascade to produce an increase in intracellular Ca(2+) concentration. Here, we used whole-cell and inside-out patch-clamp analysis to provide a functional characterization of currents activated by Ca(2+) in isolated mouse vomeronasal sensory neurons in the absence of intracellular K(+). In whole-cell recordings, the average current in 1.5 µM Ca(2+) and symmetrical Cl(-) was -382 pA at -100 mV. Ion substitution experiments and partial blockade by commonly used Cl(-) channel blockers indicated that Ca(2+) activates mainly anionic currents in these neurons. Recordings from inside-out patches from dendritic knobs of mouse vomeronasal sensory neurons confirmed the presence of Ca(2+)-activated Cl(-) channels in the knobs and/or microvilli. We compared the electrophysiological properties of the native currents with those mediated by heterologously expressed TMEM16A/anoctamin1 or TMEM16B/anoctamin2 Ca(2+)-activated Cl(-) channels, which are...Continue Reading

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Citations

Dec 25, 2015·Pflügers Archiv : European journal of physiology·Karl KunzelmannRainer Schreiber
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Dec 17, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·John MalyszGianrico Farrugia
Jun 22, 2018·Life Sciences·Kyoung Hee SeoSung Hoon Lee
Apr 20, 2021·Cellular and Molecular Life Sciences : CMLS·Raghu Ram Katreddi, Paolo E Forni
Aug 28, 2021·International Journal of Molecular Sciences·Stefano StabiliniSimone Pifferi

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