Conditional loss-of-myosin-II-function mutants reveal a position in the tail that is critical for filament nucleation

Molecular Cell
S L Moores, J A Spudich

Abstract

Myosin-II must be assembled into filaments to perform its cellular functions. Two conditional loss-of-myosin-II-function mutants were recovered from a previous genetic screen with defects that were mapped to the coiled-coil tail region of Dictyostelium myosin-II. Strikingly, both tail mutations affected the same arginine residue at position 1880. A single amino acid substitution, R1880P, disrupted both the dimerization and tetramerization steps of filament nucleation. Even a single charge reversal at this position, R1880D, was sufficient to inhibit filament assembly, while other single charge reversals in the region of antiparallel contract suppressed these filament assembly mutants. The considerable impact of small electrostatic forces on nucleation suggests that these steps are delicately balanced and easily reversible.

References

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Citations

Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·W Liang, J A Spudich
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·J H Zang, J A Spudich
Aug 1, 2015·Journal of the Royal Society, Interface·Krithika MohanPablo A Iglesias
Sep 12, 2008·Journal of Muscle Research and Cell Motility·Peggy M McMahonSarah E Rice
Mar 21, 2000·Biochimica Et Biophysica Acta·J R Sellers

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