Conditional Wwox deletion in mouse mammary gland by means of two Cre recombinase approaches.

PloS One
Brent W FergusonC M Aldaz

Abstract

Loss of WWOX expression has been reported in many different cancers including breast cancer. Elucidating the function of this gene in adult tissues has not been possible with full Wwox knockout models. Here we characterize the first conditional models of Wwox ablation in mouse mammary epithelium utilizing two transgenic lines expressing Cre recombinase, keratin 5-Cre (BK5-Cre) and MMTV-Cre. In the BK5-Cre model we observed very efficient Wwox ablation in KO mammary glands. However, BK5-Cre Wwox KO animals die prematurely for unknown reasons. In the MMTV-Cre model we observed significant ablation of Wwox in mammary epithelium with no effect on survival. In both of these models we found that Wwox deletion resulted in impaired mammary branching morphogenesis. We demonstrate that loss of Wwox is not carcinogenic in our KO models. Furthermore, no evidence of increase proliferation or development of premalignant lesions was observed. In none of the models did loss of a single Wwox allele (i.e. haploinsufficiency) have any observable phenotypic effect in mammary gland. To better understand the function of Wwox in the mammary gland, transcriptome profiling was performed. We observed that Wwox ablation results in the deregulation of gen...Continue Reading

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Citations

Sep 24, 2014·Cellular and Molecular Life Sciences : CMLS·Rami I AqeilanMohammad Abu-Odeh
Dec 21, 2012·Journal of Cellular Physiology·Suhaib K AbdeenRami I Aqeilan
Sep 24, 2015·Genes, Chromosomes & Cancer·Amanda ChooRobert I Richards
Jun 17, 2014·Biochimica Et Biophysica Acta·C Marcelo AldazMartin C Abba
Jan 23, 2018·Journal of Cell Communication and Signaling·Mai N Tran, Celina G Kleer
Jan 24, 2018·Journal of Crohn's & Colitis·Marijn C VisschedijkEleonora A M Festen
Aug 8, 2018·Cell Death & Disease·Suhaib K AbdeenRami I Aqeilan
Oct 24, 2018·Genes, Chromosomes & Cancer·Tabish HussainC Marcelo Aldaz
May 11, 2019·Cell Cycle·Suhaib K Abdeen, Rami I Aqeilan
Dec 30, 2014·Experimental Biology and Medicine·Morgan S Schrock, Kay Huebner
Apr 18, 2020·Signal Transduction and Targeted Therapy·Saleh KhawaledRami I Aqeilan
Aug 30, 2018·Nature Communications·Renxu ChangLixing Zhan
Jan 9, 2019·Frontiers in Oncology·Abrar Jamous, Zaidoun Salah
Oct 30, 2018·Frontiers in Oncology·Mayur Tanna, Rami I Aqeilan
Dec 11, 2014·Experimental Biology and Medicine·Muhannad Abu-Remaileh, Rami I Aqeilan
Jul 26, 2019·International Journal of Molecular Sciences·Yuki TochigiHiroetsu Suzuki
Nov 30, 2014·Experimental Biology and Medicine·Shin-Yi LiuYu-Jen Chen
Sep 14, 2018·Frontiers in Oncology·Karolina PospiechAndrzej K Bednarek
Nov 17, 2020·Frontiers in Cell and Developmental Biology·Ying-Tsen ChouLi-Jin Hsu
May 6, 2021·Cells·Karim TaouisFrançois Lallemand
Jun 11, 2021·Oncology Reports·Valeria Alejandra FerrettiMartín Carlos Abba
Nov 9, 2021·EMBO Molecular Medicine·Srinivasarao RepudiRami I Aqeilan

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Datasets Mentioned

BETA
GSE36665

Methods Mentioned

BETA
transgenic
PCR
chip
nuclear translocation

Software Mentioned

REViGO
DAVID
R
Bioconductor
MultiExperiment Viewer
simpleaffy
Limma
ScanScope
MeV

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