Conditionally Immortalized Neural Cell Lines: Potential Models for the Study of Neural Cell Function

Methods : a Companion to Methods in Enzymology
E R BongarzoneA T Campagnoni

Abstract

Studies on primary cell cultures have contributed significantly to our understanding of neural cell function. Nevertheless, for many studies the value of these primary cell cultures has been limited by the time the cultures survive in vitro, the quantity of cellular material available for analysis, and the need to prepare the cells on a regular basis from fresh tissue. Techniques for immortalizing cells have existed for some time, but the repertoire of immortalizing genes has grown significantly. This has expanded our ability to generate useful cell lines of specific neural types that are better models of the in vivo phenotype than previously. The constitutive expression of oncogenes keeps cells in a proliferative state that could lead to the loss of differentiated gene expression and function. An appealing improvement of immortalization methodology is the use of temperature-sensitive oncogenes that generate cell lines that can proliferate at a permissive temperature and "differentiate" at a nonpermissive temperature. The proliferation of such conditionally immortalized cell lines can be suppressed simply by increasing the temperature. Cell lines maintained at the nonpermissive temperature can enter into a stage in which they e...Continue Reading

Citations

Mar 5, 2011·Acta Neuropathologica·Ludovico Cantuti CastelvetriErnesto Roque Bongarzone
Oct 17, 2002·Journal of Neurobiology·Marcelo N Rivolta, Matthew C Holley
Apr 1, 2009·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Kerry Thompson
Sep 11, 2001·Journal of Neuroscience Research·E R BongarzoneA T Campagnoni
Oct 23, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·G Lawoko-KeraliM C Holley
Mar 18, 2005·Journal of Neuroscience Research·L S DeBruinG Harauz
Jan 27, 2004·Journal of Neuroscience Research·Augustine O FernandesAnthony T Campagnoni
Jun 8, 2007·Genesis : the Journal of Genetics and Development·Aurora BadaloniG Giacomo Consalez
May 30, 2006·The Journal of Gene Medicine·D DolcettaE Bongarzone
Apr 8, 2011·Journal of Neuroscience Research·Glauber B PereiraPatricia A Wight
Feb 14, 2012·Neurobiology of Disease·Ludovico Cantuti-CastelvetriErnesto R Bongarzone
Mar 9, 2004·Mechanisms of Development·Grace Lawoko-KeraliMatthew C Holley
Dec 9, 2003·The Journal of Biological Chemistry·Pavan K Krishnamurthy, Gail V W Johnson
Mar 24, 2015·PLoS Neglected Tropical Diseases·Caitlin A O'BrienRoy A Hall

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