Conditionally lethal Escherichia coli murein mutants contain point defects that map to regions conserved among murein and folyl poly-gamma-glutamate ligases: identification of a ligase superfamily

Biochemistry
S S EvelandM S Anderson

Abstract

Bacterial peptidoglycan biosynthesis includes four enzymatic reactions in which successive amino acid residues are ligated to uridine diphospho-N-acetylmuramic acid (UDP-MurNAc). By comparing the amino acid sequences of MurC, -D, -E, and -F proteins from various bacterial genera, four regions of homology were identified. A profile search of Swissprot for related sequences revealed that these regional similarities were present in the folyl-gamma-polyglutamate ligases. These sequence homologies appear to track with catalytic function: both enzyme families proceed through an ordered kinetic mechanism and form product via an acyl phosphate intermediate. Two highly conserved residues in region II were examined through site-directed mutagenesis of the murein D-alanyl-D-alanine-adding enzyme from Escherichia coli (murF; E158 and H188). All mutations were highly detrimental to activity with enzyme specific activity reductions of 200-4500-fold, validating the critical nature of these residues. DNA sequence analysis from three E. coli mutants harboring the murC3 (G344D), murE1 (G344K, A495S), and murF2 (A288T) mutations revealed the presence of point mutation(s) closely associated with the fourth of these aligned regions. The murF2 allel...Continue Reading

Citations

Jun 15, 2011·Journal of Molecular Modeling·Tihomir TomašićLucija Peterlin Mašič
Oct 22, 2003·Journal of Molecular Biology·Detlef D LeipeL Aravind
Mar 12, 2003·Bioorganic & Medicinal Chemistry·Ahmed El ZoeibyRoger C Levesque
Mar 6, 1999·Current Opinion in Microbiology·I Chopra
Jun 29, 2010·Nature Reviews. Microbiology·Bernd H A Rehm
May 28, 2009·Antimicrobial Agents and Chemotherapy·Ellen Z BaumKaren Bush
Nov 24, 2005·Antimicrobial Agents and Chemotherapy·Julian Gregston HurdleIan Chopra
Jul 9, 2004·Applied and Environmental Microbiology·Makoto AshiuchiHaruo Misono
Jan 15, 2011·Clinical Microbiology Reviews·Lynn L Silver
Dec 26, 2001·Journal of Bacteriology·Yuji UrushibataYasutaka Tahara
Jan 2, 2004·Journal of Bacteriology·Melissa DrysdaleTheresa M Koehler
Jun 2, 2009·Canadian Journal of Microbiology·Thomas CandelaAgnès Fouet
Dec 23, 2008·BMC Biochemistry·Catherine Paradis-BleauRoger C Levesque
Jul 25, 2006·Bioscience, Biotechnology, and Biochemistry·Makoto AshiuchiHaruo Misono
Jun 6, 2012·Annual Review of Biochemistry·Andrew L LoveringNatalie C J Strynadka
Aug 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Hong LiRobert H White
Jul 3, 2013·Natural Product Reports·Yoshimitsu HamanoKuniki Kino
Nov 24, 1999·DNA Sequence : the Journal of DNA Sequencing and Mapping·A M Lowe, R L Deresiewicz
Jun 19, 2012·Biochemical Pharmacology·Hélène BarreteauDidier Blanot
Nov 19, 2008·Bioorganic & Medicinal Chemistry Letters·Tihomir TomasićLucija Peterlin Masic
Aug 28, 2007·Biochemical and Biophysical Research Communications·Makoto AshiuchiHaruo Misono
May 13, 2006·Molecular Microbiology·Thomas Candela, Agnès Fouet
Nov 27, 2007·The Plant Journal : for Cell and Molecular Biology·Marlon GarciaHiroyoshi Takano
Sep 3, 2010·Biotechnology and Bioengineering·Daisuke YamashiroMakoto Ashiuchi
Aug 19, 2014·Molecular Microbiology·Imène KouidmiCatherine Paradis-Bleau
Dec 22, 2009·ChemMedChem·Tihomir TomasićLucija Peterlin Masic
Aug 29, 2006·Journal of Molecular Biology·Clyde A Smith

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Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.