Conditioned medium from fresh and demineralized bone enhances osteoclastogenesis in murine bone marrow cultures

Clinical Oral Implants Research
Jordi Caballé-SerranoReinhard Gruber

Abstract

Osteoclasts rapidly form on the surface of bone chips at augmentation sites. The underlying molecular mechanism, however, is unclear. Soluble factors released from bone chips in vitro have a robust impact on mesenchymal cell differentiation. Whether these soluble factors change the differentiation of hematopoietic cells into osteoclasts remains unknown. Osteoclastogenesis, the formation of tartrate-resistant acid phosphatase-positive multinucleated cells, was studied with murine bone marrow cultures exposed to RANKL and M-CSF, and conditioned medium from fresh (BCM) and demineralized bone matrix (DCM). Histochemical staining, gene and protein expression, as well as viability assays were performed. This study shows that BCM had no impact on osteoclastogenesis. However, when BCM was heated to 85°C (BCMh), the number of tartrate-resistant acid phosphatase-positive multinucleated cells that developed in the presence of RANKL and M-CSF approximately doubled. In line with the histochemical observations, there was a trend that BCMh increased expression of osteoclast marker genes, in particular the transcription factor c-fos. The expression of c-fos was significantly reduced by the TGF-β receptor I antagonist SB431542. DCM significantl...Continue Reading

References

Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·W HofstetterC Mueller
Oct 1, 1996·Journal of Mammary Gland Biology and Neoplasia·M H Barcellos-Hoff
Feb 15, 2001·Microscopy Research and Technique·K KoliJ Keski-Oja
Jun 8, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T KonT A Einhorn
Aug 7, 2002·Biochemical and Biophysical Research Communications·Florence LorgetElizabeth Offord
Sep 6, 2003·Journal of Clinical Periodontology·G CardaropoliJ Lindhe
Sep 13, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·L C GerstenfeldT A Einhorn
Aug 17, 2004·Critical Reviews in Clinical Laboratory Sciences·Marko HyytiäinenJorma Keski-Oja
Apr 4, 2007·The Journal of Biological Chemistry·Sudarshana M SharmaMichael C Ostrowski
Dec 7, 2007·Biochemical and Biophysical Research Communications·S W FoxA C Lovibond
Jul 14, 2009·Bone·Claire JacquinSun-Kyeong Lee
Sep 13, 2011·Nature Medicine·Jinhu XiongCharles A O'Brien
Sep 13, 2011·Nature Medicine·Tomoki NakashimaHiroshi Takayanagi
Dec 14, 2011·The Journal of Bone and Joint Surgery. American Volume·Chad Myeroff, Michael Archdeacon
Jan 20, 2012·Nature·Gérard Karsenty, Mathieu Ferron
Feb 18, 2012·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Hiroya MiyamotoTakeshi Miyamoto
Jul 10, 2012·American Journal of Respiratory Cell and Molecular Biology·Malgorzata WygreckaPhilipp Markart
Dec 19, 2012·Experimental Cell Research·Woo-Shin KimHong-Hee Kim
Jan 3, 2013·The Journal of the American Academy of Orthopaedic Surgeons·Gregory Grabowski, Chris A Cornett
Jan 26, 2013·Clinical Oral Implants Research·Mark Farmer, Ivan Darby
Mar 16, 2013·Clinical Implant Dentistry and Related Research·Mahmoud KazemiVahid Rakhshan
May 25, 2013·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Hermann AgisReinhard Gruber
Oct 26, 2013·Journal of Dental Research·D BuserU C Belser
Jan 28, 2014·Clinical Implant Dentistry and Related Research·Jianbo PengReinhard Gruber
Feb 1, 2014·International Journal of Oral and Maxillofacial Surgery·U KuchlerR Gruber
Feb 4, 2014·The Journal of Clinical Investigation·Janet L Crane, Xu Cao
Jun 4, 2014·Clinical Oral Implants Research·Eliane BroleseReinhard Gruber
Sep 13, 2014·The International Journal of Oral & Maxillofacial Implants·Jordi Caballé-SerranoReinhard Gruber
Mar 20, 2015·Journal of Periodontology·Guenther Schuldt FilhoReinhard Gruber

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Citations

Sep 2, 2020·Clinical Oral Investigations·Ludovica ParisiMaria B Asparuhova
Jun 14, 2018·International Journal of Oral Science·Maria B AsparuhovaVivianne Chappuis
Dec 14, 2016·Acta orthopaedica et traumatologica turcica·Chengxue WangJieping Cheng

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