Conditioned medium from gerbil--mouse T cell heterohybridomas improved antibody secretion

Experimental Animals
Takao UkajiOsamu Kai

Abstract

Mongolian gerbils (Meriones unguiculatus) are widely used as animal models for a variety of infectious diseases. However, immunological reagents such as cytokines have not been characterized. Two heterohybridomas, D9(E6)C2B3 and D9(E4), obtained by fusion of gerbil splenocytes with mouse myeloma cells (P3-X63-Ag8.653), expressed gerbil CD3G mRNA. These cells were suggested to be T cell heterohybridomas. They also expressed gerbil IL6 [D9(E6)C2B3] and TGFB [D9(E4) and D9(E6)C2B3] mRNAs. The addition of conditioned medium (CM) obtained from the culture of D9(E6)C2B3 significantly enhanced antibody secretion and expression of gerbil Cγ1 and Cε IGHC mRNAs in the B11D2(C2) heterohybridoma, which secretes gerbil IgG1. However, the addition of CM from both heterohybridomas did not improve in proliferation of B11D2(C2) cells. These results indicate that CM from D9(E6)C2B3 improved the culture of gerbil-mouse heterohybridomas, possibly by secreting gerbil IL6.

References

Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J Van SnickR J Simpson
Apr 1, 1985·Molecular Immunology·M C KuoT J Kindt
Mar 27, 2004·The Journal of Biological Chemistry·Hiroyuki HoriuchiHaruo Matsuda
May 4, 2005·Pharmacology & Therapeutics·Jaime ImitolaSamia J Khoury
May 25, 2005·Veterinary Immunology and Immunopathology·Norihisa NishimichiHaruo Matsuda
Sep 29, 2011·Animal Science Journal = Nihon Chikusan Gakkaihō·Takao UkajiOsamu Kai
Sep 15, 2012·Journal of Immunological Methods·Takao Ukaji, Osamu Kai

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Datasets Mentioned

BETA
AB177841
AB663133

Methods Mentioned

BETA
protein assay
PCR
ELISA

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