Conditioned medium from relapsing-remitting multiple sclerosis patients reduces the expression and release of inflammatory cytokines induced by LPS-gingivalis in THP-1 and MO3.13 cell lines

Cytokine
Patrizia BalleriniOriana Trubiani

Abstract

The present research was aimed at evaluating the effect of the conditioned medium (CM) from human periodontal ligament stem cells (hPDLSCs) obtained from healthy donors (hPDLSCs-CM) and from Relapsing-Remitting Multiple Sclerosis patients (RR-MS-CM) on inflammatory response induced by Porphyromonas gingivalis lipopolysaccharide (LPS-G) in a monocytoid human cell line (THP-1) and human oligodendrocyte cell line (MO3.13). Human periodontal ligament biopsies were carried out from control donor patients and selected RR-MS donors. Sample tissues were obtained from premolar teeth during root scaling and subsequently cultured. The effect of hPDLSCs-CM and RR-MS-CM on cell viability in PMA differentiated THP-1 (as a model of microglia) was measured using a 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assay. The same experiments were performed in undifferentiated and differentiated MO3.13 cells used as models of progenitor cells and oligodendrocytes, respectively. The expression of tumor necrosis factor alpha (TNF)-α, interleukin (IL)-1β and IL-6 was evaluated by Real-Time Polymerase Chain Reaction (RT-PCR), and enzyme-linked immunosorbent assay (ELISA). The expression level of the Toll-like receptor 4 (TLR-4), for...Continue Reading

Citations

Jan 24, 2018·International Journal of Molecular Sciences·Francesca DiomedeOriana Trubiani
Mar 30, 2018·International Journal of Molecular Sciences·Francesca DiomedeEmanuela Mazzon
Apr 25, 2019·Stem Cells and Development·Oriana TrubianiFrancesca Diomede
Jan 5, 2019·Current Stem Cell Research & Therapy·Tatjana KanjevacDarko Bosnakovski
Jul 19, 2019·Materials·Francesca DiomedeJacopo Pizzicannella
Nov 5, 2019·Stem Cell Reviews and Reports·Bruna SinjariSergio Caputi
Aug 12, 2018·International Journal of Molecular Sciences·Veronica Lanza CariccioEmanuela Mazzon
May 8, 2020·International Journal of Molecular Sciences·Francesca DiomedeOriana Trubiani
Sep 23, 2020·International Journal of Molecular Sciences·Francesca DiomedeOriana Trubiani
Jan 13, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yomaira L UscáteguiManuel F Valero
Feb 23, 2019·International Journal of Immunopathology and Pharmacology·Sergio CaputiVladimir Khavinson
Mar 27, 2018·Biological Reviews of the Cambridge Philosophical Society·Douglas B Kell, Etheresia Pretorius
Jun 16, 2018·Scientific Reports·Letizia RomeoEmanuela Mazzon
Dec 20, 2018·International Journal of Molecular Sciences·Jacopo PizzicannellaFrancesca Diomede
Feb 6, 2021·Oxidative Medicine and Cellular Longevity·Guya D MarconiFrancesca Diomede
Aug 14, 2021·Journal of Periodontal & Implant Science·Alondra Del Carmen Ruíz-GutiérrezCelia Guerrero-Velázquez
Jan 20, 2021·ACS Biomaterials Science & Engineering·Yuqing WangYubo Fan

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